中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2011年
5期
345-348
,共4页
滕晓英%王鹏%周新刚%沈冰%孙磊%郎振为
滕曉英%王鵬%週新剛%瀋冰%孫磊%郎振為
등효영%왕붕%주신강%침빙%손뢰%랑진위
肝炎病毒,乙型%免疫组织化学%程序性死亡分子-1
肝炎病毒,乙型%免疫組織化學%程序性死亡分子-1
간염병독,을형%면역조직화학%정서성사망분자-1
Hepatitis B virus%Immunohistochemistry%Programmed death 1
目的 通过比较慢性HBV感染免疫耐受期和免疫清除期的患者肝组织中程序性死亡分子-1及其配体的表达情况,探讨其与机体免疫功能状态的关系.方法 收集肝组织活体检查标本并分为免疫清除期组25例、免疫耐受期组19例,用免疫组织化学方法检测标本汇管区中T淋巴细胞程序性死亡分子-1及其配体的表达情况,通过半定量评分系统计算其占CD3阳性细胞的百分数,用t检验比较两组病例间程序性死亡分子-1及其配体表达的差异.结果 免疫耐受期组肝组织汇管区T淋巴细胞中程序性死亡分子-1所占CD3阳性细胞比率为63.79%±6.94%,高于免疫清除期的54.36%±10.08%,两组比较,t=3.492,P<0.01,差异有统计学意义;程序性死亡分子配体-1于T淋巴细胞中的表达在免疫耐受期组(66.47%±8.40%)中高于免疫清除期组(52.64%±6.20%),两组比较,t=6.288,P<0.01,差异有统计学意义.程序性死亡分子配体-1在枯否细胞中的表达强度及范围在两组间差异无统计学意义(P>0.05).结论 慢性HBV感染者肝组织中的程序性死亡分子-1及其配体表达水平的差异反映了免疫耐受期和免疫清除期的不同免疫功能状态.
目的 通過比較慢性HBV感染免疫耐受期和免疫清除期的患者肝組織中程序性死亡分子-1及其配體的錶達情況,探討其與機體免疫功能狀態的關繫.方法 收集肝組織活體檢查標本併分為免疫清除期組25例、免疫耐受期組19例,用免疫組織化學方法檢測標本彙管區中T淋巴細胞程序性死亡分子-1及其配體的錶達情況,通過半定量評分繫統計算其佔CD3暘性細胞的百分數,用t檢驗比較兩組病例間程序性死亡分子-1及其配體錶達的差異.結果 免疫耐受期組肝組織彙管區T淋巴細胞中程序性死亡分子-1所佔CD3暘性細胞比率為63.79%±6.94%,高于免疫清除期的54.36%±10.08%,兩組比較,t=3.492,P<0.01,差異有統計學意義;程序性死亡分子配體-1于T淋巴細胞中的錶達在免疫耐受期組(66.47%±8.40%)中高于免疫清除期組(52.64%±6.20%),兩組比較,t=6.288,P<0.01,差異有統計學意義.程序性死亡分子配體-1在枯否細胞中的錶達彊度及範圍在兩組間差異無統計學意義(P>0.05).結論 慢性HBV感染者肝組織中的程序性死亡分子-1及其配體錶達水平的差異反映瞭免疫耐受期和免疫清除期的不同免疫功能狀態.
목적 통과비교만성HBV감염면역내수기화면역청제기적환자간조직중정서성사망분자-1급기배체적표체정황,탐토기여궤체면역공능상태적관계.방법 수집간조직활체검사표본병분위면역청제기조25례、면역내수기조19례,용면역조직화학방법검측표본회관구중T림파세포정서성사망분자-1급기배체적표체정황,통과반정량평분계통계산기점CD3양성세포적백분수,용t검험비교량조병례간정서성사망분자-1급기배체표체적차이.결과 면역내수기조간조직회관구T림파세포중정서성사망분자-1소점CD3양성세포비솔위63.79%±6.94%,고우면역청제기적54.36%±10.08%,량조비교,t=3.492,P<0.01,차이유통계학의의;정서성사망분자배체-1우T림파세포중적표체재면역내수기조(66.47%±8.40%)중고우면역청제기조(52.64%±6.20%),량조비교,t=6.288,P<0.01,차이유통계학의의.정서성사망분자배체-1재고부세포중적표체강도급범위재량조간차이무통계학의의(P>0.05).결론 만성HBV감염자간조직중적정서성사망분자-1급기배체표체수평적차이반영료면역내수기화면역청제기적불동면역공능상태.
Objective To detect and compare the PD- 1/PD-L1 (programmed death 1/programmed death 1 ligand) expressions in the liver tissues of chronic HBV infection patients in immune tolerant phase and those in immune clearance phase. Methods Liver biopsy samples were divided into two groups: 25 samples from patients in immune clearance phase and 19 samples from patients in immune tolerant phase.PD-1/PD-L1 expressions on T lymphocytes in these liver biopsy specimens were detected by immunobis tochemistry method. Percentage of PD-1/PD-L1 positive cells among CD3 positive cells was calculated by semi-quantitative evaluation. Differences between the two groups were statistically analyzed. Results PD1/PD-L1 expressions were significantly higher in the patients in immune tolerant phase as compared to that in immune active phase (P < 0.05). No statistical difference found between the two groups for PD-L1 expression in Kupffer cells (P > 0.05). Conclusion PD-1/PD-L1 expression level can reflect the immune functions of chronic hepatitis B patients.
