CAJ | 학술논문

目的探讨不同频率次声辐射对小鼠睾丸组织的病理学损伤作用以及连环蛋白(β-catenin)与睾丸损伤间的关系.方法采用随机数字表法将60只成年BALB/C小鼠分为对照组(6只)和次声组(54只),次声组小鼠又根据次声作用频率不同细分为3个亚组,每组18只,分别施以4 Hz、8 Hz及20 Hz次声干预(次声声压级均为130 dB),持续作用2h;对照组小鼠则于相同时间点置于次声舱内,但期间无次声能量输出.各组分别于次声辐射停止后3h、9h和21 h时各取6只小鼠处死(对照组则于上述时间点各取2只处死),提取睾丸组织固定并进行HE染色,观察小鼠睾丸组织病理损伤情况；采用免疫组化技术观察次声作用停止3h后各组小鼠睾丸细胞 β-catenin表达情况.结果 8 Hz-130 dB次声辐射对小鼠睾丸的病理损伤作用最显著,可见小鼠精原细胞、精母细胞出现不同程度水肿,细胞结构松散,细胞间连接减少或消失,精子形态受损,并以次声辐射停止3h后病理损伤程度最严重.免疫组化检查结果显示,次声辐射停止3h后,对照组及各次声亚组β-catenin均主要存在于小鼠睾丸间质细胞中,如支持细胞和肌上皮细胞胞膜中均可见强阳性表达,其表达水平与生精细胞周期无关,且不同频率次声亚组β-catenin表达强度无明显差异.结论 4 Hz-130 dB和8 Hz-130 dB次声辐射均可引起小鼠睾丸细胞形态受损,20 Hz-130 dB次声辐射则对小鼠睾丸组织无明显损伤作用；次声辐射对小鼠睾丸组织β-catenin表达无明显影响,其损伤效应可能是由次声产生的机械力所致.
목적 탐토불동빈솔차성복사대소서고환조직적병이학손상작용이급련배단백(β-catenin)여고환손상간적관계.방법 채용수궤수자표법장60지성년BALB/C소서분위대조조(6지)화차성조(54지),차성조소서우근거차성작용빈솔불동세분위3개아조,매조18지,분별시이4 Hz、8 Hz급20 Hz차성간예(차성성압급균위130 dB),지속작용2h;대조조소서칙우상동시간점치우차성창내,단기간무차성능량수출.각조분별우차성복사정지후3h、9h화21 h시각취6지소서처사(대조조칙우상술시간점각취2지처사),제취고환조직고정병진행HE염색,관찰소서고환조직병리손상정황；채용면역조화기술관찰차성작용정지3h후각조소서고환세포 β-catenin표체정황.결과 8 Hz-130 dB차성복사대소서고환적병리손상작용최현저,가견소서정원세포、정모세포출현불동정도수종,세포결구송산,세포간련접감소혹소실,정자형태수손,병이차성복사정지3h후병리손상정도최엄중.면역조화검사결과현시,차성복사정지3h후,대조조급각차성아조β-catenin균주요존재우소서고환간질세포중,여지지세포화기상피세포포막중균가견강양성표체,기표체수평여생정세포주기무관,차불동빈솔차성아조β-catenin표체강도무명현차이.결론 4 Hz-130 dB화8 Hz-130 dB차성복사균가인기소서고환세포형태수손,20 Hz-130 dB차성복사칙대소서고환조직무명현손상작용；차성복사대소서고환조직β-catenin표체무명현영향,기손상효응가능시유차성산생적궤계력소치.
Objective To investigate the effects of infrasound at different frequencies on the pathological morphology of mouse testes and to explore the possible involvement of β-cateinin in the mechanism of injury. Methods Sixty adult BALB/C mice were randomly divided into 2 groups.One group of 6 mice were the controls without infrasound exposure.The other group with 54 mice was evenly divided into 3 subgroups of 18 exposed to 130 dB infrasound at 4 Hz,8 Hz or 20 Hz for 2 hours daily.The pathological morphology of the mice's testes was observed 3,9 and 21 h after exposure.Immunohistochemical staining was used to examine β-catenin expression 3 h after exposure.Results Marked damage was observed in the morphology of the testicular cells of rats in the 8 Hz group.Cell denaturation was found in germinal cells.The cell structure was loosened and the cell junctions had decreased or disappeared.The damage was most serious at 3 h after exposure.But at the same time point,pathological damage of testicular cells was not obvious in the 4 Hz group,with only a few testes cells swollen.The morphology of testicular tissue of mice in the 20 Hz subgroup exhibited no significant difference from the control group.β-catenin was mainly found in the membranes of Leydig cells,Sertoli cells and myoepithelial cells in all of the mice,and there was no significant change in the expression level or distribution pattern between the exposed mice and the controls.No expression ofβ-catenin was found in spermatogenic cells. Conclusion Infrasound at 4 Hz or 8 Hz can damage testicular tissue,at least in mice.At 20 Hz there is no significant effect on the testes.The loosened cell structure might have been caused by the mechanical force produced by the infrasound and not by any decrease of β-catenin.