CAJ | 학술논문

目的研究结缔组织生长因子(CTGF)在兔眼小梁切除术后滤过泡中的表达及意义.方法对照实验研究.将49只健康家兔分为5组:正常眼未手术组(A组),高眼压未手术组(B组),高眼压模型小梁切除术组(C组),正常眼小梁切除术组(D组),正常眼假手术组(E组),并分别在术后第2、5、7、14及21天,取右眼滤过泡部位的球结膜和浅层巩膜组织,A组和B组作为对照组.采用RT-PCR法检测CTGF mRNA的相对表达量,应用免疫组织化学方法检测CTGF蛋白表达水平,HE染色行组织病理学检查.采用SAS 9.1.3统计学软件,对逆转录聚合酶链反应(RT-PCR)和免疫组织化学检测结果采用析因设计定量资料方差分析.结果 C、D、E组术后CTGF表达水平高于A组和B组,术后第5天CTGF表达水平达到高峰,与其他时间点的表达差异有统计学意义(F=19.54,P<0.05);术后第2和5天,CTGF mRNA表达水平,C组高于同期D组(t=2.300,5.140;P<0.05);术后第2、5、7、14天,D组高于同期E组(t=-2.927,-6.424,-4.176,-4.997,P<0.05).CTGF蛋白表达水平,术后第2、5、7、14天,C组高于同期D组(t=-7.147,-10.955,-9.900,-6.385,P<0.05);术后第2、5、7、14天,D组高于同期E组(F=68.33,P<0.05).组织病理学检查结果显示,术后第5天,C、D、E组局部炎性反应达高峰,表现为中性粒细胞、单核细胞及巨噬细胞浸润,成纤维细胞增殖达高峰.结论小梁切除术后家兔CTGF表达水平的变化趋势表明,CTGF在伤口愈合及瘢痕形成过程中可能起重要作用,高眼压状态可促进CTGF表达水平上调.(手华眼科杂志,2009,45:168-174)
목적 연구결체조직생장인자(CTGF)재토안소량절제술후려과포중적표체급의의.방법 대조실험연구.장49지건강가토분위5조:정상안미수술조(A조),고안압미수술조(B조),고안압모형소량절제술조(C조),정상안소량절제술조(D조),정상안가수술조(E조),병분별재술후제2、5、7、14급21천,취우안려과포부위적구결막화천층공막조직,A조화B조작위대조조.채용RT-PCR법검측CTGF mRNA적상대표체량,응용면역조직화학방법 검측CTGF단백표체수평,HE염색행조직병이학검사.채용SAS 9.1.3통계학연건,대역전록취합매련반응(RT-PCR)화면역조직화학검측결과 채용석인설계정량자료방차분석.결과 C、D、E조술후CTGF표체수평고우A조화B조,술후제5천CTGF표체수평체도고봉,여기타시간점적표체차이유통계학의의(F=19.54,P<0.05);술후제2화5천,CTGF mRNA표체수평,C조고우동기D조(t=2.300,5.140;P<0.05);술후제2、5、7、14천,D조고우동기E조(t=-2.927,-6.424,-4.176,-4.997,P<0.05).CTGF단백표체수평,술후제2、5、7、14천,C조고우동기D조(t=-7.147,-10.955,-9.900,-6.385,P<0.05);술후제2、5、7、14천,D조고우동기E조(F=68.33,P<0.05).조직병이학검사결과 현시,술후제5천,C、D、E조국부염성반응체고봉,표현위중성립세포、단핵세포급거서세포침윤,성섬유세포증식체고봉.결론 소량절제술후가토CTGF표체수평적변화추세표명,CTGF재상구유합급반흔형성과정중가능기중요작용,고안압상태가촉진CTGF표체수평상조.(수화안과잡지,2009,45:168-174)
Objective To investigate the role of connective tissue growth factor (CTGF) after trabeculectomy associated with wound healing and to identify the role of CTGF in this process. Methods It was a experimental study. Forty-nine rabbits were used and divided into 5 groups: normal eyes without trabeculectomy group (group A), ocular hypertension (OHT) model without trabeculectomy group (group B), OHT model with trabeculectomy group (group C), normal eyes with trabeculectomy group (group D) and normal eyes with sham operation group (group E). Group A and B were as control CTGF mRNA was detected by RT-PCR using blebs and tissues harvested at day 2, 5, 7, and 14. Three replicates of three blebs per time point in the right eyes were collected. The expression of CTGF protein was detected by immunohistochemistry and inflammatory histopathology was inspected by HE staining using the whole eyeball harvested in the left eyes. Results Compared to group A and B, the expression of CTGF was significantly increased at day 5 after surgery (F=19.54, P<0.05) in group C, D, and E. The expression of CTGF mRNA in group C is significantly higher than that in group D at day 2 and 5 (t=2.300,5.140,P <0.05), while group D is significantly higher than that in group E at day 2, 5, 7, and 14 (t=-2.927, -6.424, -4.176, -4.997, P<0.05). The expression of CTGF protein in group C is significantly higher than that in group D (t=-7.147, -10.955, -9.900, -6.385, P<0.05), and group D is higher than that in group E (F=68.33,P<0.05) at day 2, 5, 7, and 14, respectively. Inflammatory reaction reached peak at day 5 after surgery in group C, D, and E showing an infiltration of neutrophil, monocytes, macrophagus, and the proliferation of fibroblast. Conclusions Overexpression of CTGF in the blebs after trabeculectomy demonstrates that CTGF may play an important role in the process of wound healing. Furthermore, ocular hypertension may be involved in the upregulation of CTGF expression. (Chin J Ophthalmol, 2009,45:168-174)