CAJ | 학술논문

目的：通过药物抑制乙肝病毒血清标志物(HBV-M)的实验，观察药物抑制HBV的作用。方法：先将药物做初筛试验，再对选择出的药物进行酶抑制试验和对照试验。用ELISA检测各组的HBV-M光密度(OD)值。结果：经初步筛选，可见84消毒剂和藿香正气水能够使HBsAg和HBeAg的OD值明显降低。藿香正气水可使二者 OD值低于阴性界限值(Cutoff)。酶抑制试验显示其对ELISA无明显干扰。对照试验中，藿香正气水抑制HBsAg 的OD值曲线也表明其能有效抑制HBV-M的抗原性。结论：药物抑制HBV-M的实验研究，能够为筛选具有抗 HBV作用的药物提供一些依据。
목적：통과약물억제을간병독혈청표지물(HBV-M)적실험，관찰약물억제HBV적작용。방법：선장약 물주초사시험，재대선택출적약물진행매억제시험화대조시험。용ELISA검측각조적HBV-M광밀도(OD)치。결 과：경초보사선，가견84소독제화곽향정기수능구사HBsAg화HBeAg적OD치명현강저。곽향정기수가사이자 OD치저우음성계한치(Cutoff)。매억제시험현시기대ELISA무명현간우。대조시험중，곽향정기수억제HBsAg 적OD치곡선야표명기능유효억제HBV-M적항원성。결론：약물억제HBV-M적실험연구，능구위사선구유항 HBV작용적약물제공일사의거。
Objective: To investigate the effect of anti-HBV drugs using the experiment of anti-HBV-M. Methods:Drugs were se lected with screening test,and were deteced with inhibition of HRP and control test. The optical density (OD) values of HBV viral marks were determined with ELISA. Results: The OD value of HBsAg and HBe Ag in disinfectant 84 and Huc Xiang Zheng Qi Shui treatment groups decreased,and was lower than the cutoff in Huo Xiang Zheng Qi Shui treatment group. The drug did not show any obvious interference with ELISA in t he inhibition test of HRP. The curve of OD value from Huo Xiang Zheng Qi Shui inhibiting HBsAg showed that Huo Xiang Zheng Qi Shui can effectively inhibit the antigen of HBV-M by control test. Conclusion: The study of screening test of anti-HBV drugs can provide the evidence of screening anti-HBV.