CAJ | 학술논문

目的建立用高效液相色谱法测定复方制剂中戊酸雌二醇和炔诺酮含量的方法. 方法用氰基键合硅胶为固定相,乙腈―水(47∶53)为流动相,黄体酮为内标,UV检测波长为280 nm.结果该方法回收率为戊酸雌二醇100.6 %, RSD=0.9 %(n = 9),炔诺酮100.2 %, RSD = 0.8 %(n = 9);测定了三批样品,结果满意.结论该法不需经复杂的样品处理,简便、快速,准确可靠,可作为该复方制剂的含量测定方法.
목적건립용고효액상색보법측정복방제제중무산자이순화결낙동함량적방법. 방법용청기건합규효위고정상,을정―수(47∶53)위류동상,황체동위내표,UV검측파장위280 nm.결과해방법회수솔위무산자이순100.6 %, RSD=0.9 %(n = 9),결낙동100.2 %, RSD = 0.8 %(n = 9);측정료삼비양품,결과만의.결론해법불수경복잡적양품처리,간편、쾌속,준학가고,가작위해복방제제적함량측정방법.
OBJECTIVE To develop a HPLC method for determination of estradiol valerate and norethisterone in compound tablets. METHOD The chromatographic system consisted of Zorbax CN column and mobile phase of MeCN-H2O (47 : 53), with a detection wavelength at 280 nm. Progesterone was as the internal standard. RESULTS The average (n=9) recovery both for estradiol valerate and norethisterone was 100.6 % with RSD 0.9 % and 100.2 % with RSD 0.8%, respectively. Three batches of samples were analyzed, and satisfactory results were achieved. CONCLUSION Without complicated sampling procedure, the method was simple, rapid and accurate for the assay of the components in this pharmaceutical formulation.