CAJ | 학술논문

本实验主要分析青蒿素作用后p53功能突变的人乳腺癌细胞MDA-MB-435微核的变化.通过MTT法测定不同药物浓度及不同药物作用时间对细胞毒性的影响,通过胞质分裂阻滞法(CB法)计数青蒿素作用联合不同剂量~(60)Co γ射线照射后细胞的微核率(Micronucleus frequency,MNF)和微核细胞率(Micronucleus cell frequency,MNCF),将青蒿素作用的细胞与单纯照射细胞的微核率和微核细胞率的统计分析结果进行比较.实验结果表明,青蒿素对实验细胞MDA-MB-435毒性较小,在实验药物浓度为200 μmol/L,作用时间为24 h时,将CB微核法得到的数据进行回归拟合可以得到药物作用及未经药物作用的剂量效应曲线,比较两组曲线,青蒿素作用组的微核率及微核细胞率明显高于单纯照射细胞(p>0.05).
본실험주요분석청호소작용후p53공능돌변적인유선암세포MDA-MB-435미핵적변화.통과MTT법측정불동약물농도급불동약물작용시간대세포독성적영향,통과포질분렬조체법(CB법)계수청호소작용연합불동제량~(60)Co γ사선조사후세포적미핵솔(Micronucleus frequency,MNF)화미핵세포솔(Micronucleus cell frequency,MNCF),장청호소작용적세포여단순조사세포적미핵솔화미핵세포솔적통계분석결과진행비교.실험결과표명,청호소대실험세포MDA-MB-435독성교소,재실험약물농도위200 μmol/L,작용시간위24 h시,장CB미핵법득도적수거진행회귀의합가이득도약물작용급미경약물작용적제량효응곡선,비교량조곡선,청호소작용조적미핵솔급미핵세포솔명현고우단순조사세포(p>0.05).
In order to analyze the change of micronucleus in the p53 mutant human breast cancer cells MDA-MB-435 caused by artemisinin additive, MTT method was used to check the effect of different concentration and treatment time of artemisinin to cytotoxicity. Cytokinesis block micronucleus method (CB method) was used to determine the micronucleus frequency (MNF) and the micronucleus cell frequency (MNCF) of cells treated by artemisinin additive and with different doses of ~(60)Co γ-rays. The experiment results show that artemisinin is of little toxicity to MDA-MB-435. When drug concentration is 200μmol/L and treatment time is 24 h, the dose-response curves of MNF and MNCF of irradiated cells combined with treatment of or not artemisinin additive confirm that MNF and MNCF of cells treated by artemisinin are both significantly higher than that treated by radiation alone. It can be deduced that artemisinin may have some sensitive action on radiation therapy.