CAJ | 학술논문

从哥伦比亚生态型拟南(Arabidopsis thaliana ecotype Columbia)中克隆了AtCpNifS的完整CDS序列.通过测序和序列比对分析,确认了克隆序列的完整性和正确性.在此基础上,构建了该基因的原核表达载体(BL21-pET28-AtCpNifS),并从该质粒中成功分离了表达蛋白(AtCpNifS).
종가륜비아생태형의남(Arabidopsis thaliana ecotype Columbia)중극륭료AtCpNifS적완정CDS서렬.통과측서화서렬비대분석,학인료극륭서렬적완정성화정학성.재차기출상,구건료해기인적원핵표체재체(BL21-pET28-AtCpNifS),병종해질립중성공분리료표체단백(AtCpNifS).
The whole coding domain sequence(CDS)of AtCpNifS was isolated from Arabidopsis thaliana ecotype Columbia.The integrity and accuracy of cloned sequence was confirmed by sequencing and aligning.On this basis,the prokaryotic expression vector(BL21-pET28-AtCpNifS)with the gene was constructed and the expression protein(AtCpNifS)wag separated from the plasmid successfully.