CAJ | 학술논문

用SABC免疫组织化学技术,观察家兔海马各区nNOS阳性神经元在去卵巢及雌激素替代治疗后的形态结构及分布变化,为雌激素类药物防治绝经后老年性痴呆症提供理论依据.结果表明,家兔海马各区都有nNOS阳性神经元分布;去卵巢后海马nNOS阳性神经元的形态结构及分布变化有区域差异性:与假手术对照组相比,在海马CA1区、CA3区、齿状回(DG)阳性神经元数量明显减少(P<0.05),而在CA2区数量明显增多(P<0.05).CA1、CA3区和DG的阳性神经元胞体截面积明显变小,最长突起长度明显变短,第一级突起数变少,与假手术组有显著差异(P<0.05).CA2区阳性神经元胞体截面积明显变小(P<0.05),最长突起长度、第一级突起数增多,但差异不显著(P>0. 05);nNOS阳性神经元的4种指标在雌激素替代治疗组与假手术组之间无显著差异(P>0. 05).结果提示:雌激素可能通过影响海马nNOS的表达来影响脑的学习和记忆功能.
용SABC면역조직화학기술,관찰가토해마각구nNOS양성신경원재거란소급자격소체대치료후적형태결구급분포변화,위자격소류약물방치절경후노년성치태증제공이론의거.결과표명,가토해마각구도유nNOS양성신경원분포;거란소후해마nNOS양성신경원적형태결구급분포변화유구역차이성:여가수술대조조상비,재해마CA1구、CA3구、치상회(DG)양성신경원수량명현감소(P<0.05),이재CA2구수량명현증다(P<0.05).CA1、CA3구화DG적양성신경원포체절면적명현변소,최장돌기장도명현변단,제일급돌기수변소,여가수술조유현저차이(P<0.05).CA2구양성신경원포체절면적명현변소(P<0.05),최장돌기장도、제일급돌기수증다,단차이불현저(P>0. 05);nNOS양성신경원적4충지표재자격소체대치료조여가수술조지간무현저차이(P>0. 05).결과제시:자격소가능통과영향해마nNOS적표체래영향뇌적학습화기억공능.
To study the related changes of nitric oxide synthase (nNOS) positive neurons in the sections of the hippocampus of female rabbit (Sprague-Dawley strain) after ovariectomy and estrogen replacement therapy,the distribution and structure of nNOS positive neurons was observed in rabbit hippocampus using SABC immunohistochemical technique,which provides basic theories for preventing and treating Alzheimer's disease with estrogen replacement therapy.The results showed : There are comprehensive nNOS-positive staining cells in all regions of hippocampus.After ovariectomy,the changes of nNOS positive neurons in subregion of hippocampus were not equal: the number of positive neurons in subiculum CA1,CA3 and dentate gyrus(DG) were decreased compared with control group (P<0.05) ,while the number of positive neurons in subiculum CA2 was increased (P<0.05).The average section area of positive neuron was smaller and the length of the longest process become shorter and the number of first tuber turns were less in subiculum CA1,CA3 and DG than that in control group(P<0.05).The average section area of positive neuron was smaller while the length of the longest process and the number of first tuber have no remarkable change in CA2 than that in control group (P>0.05).Four kinds index of positive cells in the subregion of hippocampus showed no significant difference between estradiolreplacement therapy and the control group(P>0.05).The results indicated that estrogen maybe affect the learning and memory by influencing the expression of nNOS in hippocampus.