CAJ | 학술논문

目的探讨肺炎衣原体(chlamydia pneumoniae,CPn)感染高脂饮食小鼠的血脂、氧化修饰低密度脂蛋白(OX-LDL)及内膜损伤的变化及黄连素的干预作用.方法采用高脂饲料饲喂C57BL/6J小鼠,以肺炎衣原体滴鼻感染(隔周接种1次,共3次)的方式制作动脉粥样硬化模型.成模后的小鼠随机分为6组:黄连素早期干预组(Berl)、黄连素后期干预组(Ber2)、阿奇霉素组、立普妥组、高脂+CPn对照组、正常对照组.测定各组血清学及病理电镜检查.结果高脂+CPn感染小鼠的血脂紊乱、OX-LDL升高,并有不同程度内膜损伤,黄连素、阿奇霉素、立普妥干预组上述指标均有不同程度降低.结论黄连素具有调节Cpn感染高脂饮食小鼠血脂紊乱、保护血管内膜作用.
목적 탐토폐염의원체(chlamydia pneumoniae,CPn)감염고지음식소서적혈지、양화수식저밀도지단백(OX-LDL)급내막손상적변화급황련소적간예작용.방법 채용고지사료사위C57BL/6J소서,이폐염의원체적비감염(격주접충1차,공3차)적방식제작동맥죽양경화모형.성모후적소서수궤분위6조:황련소조기간예조(Berl)、황련소후기간예조(Ber2)、아기매소조、립보타조、고지+CPn대조조、정상대조조.측정각조혈청학급병리전경검사.결과 고지+CPn감염소서적혈지문란、OX-LDL승고,병유불동정도내막손상,황련소、아기매소、립보타간예조상술지표균유불동정도강저.결론 황련소구유조절Cpn감염고지음식소서혈지문란、보호혈관내막작용.
Objective To study the variation of the level of lipids, OX-LDL and vascular intimal injury in diet-induced hyperlipidemic and chronic Cpn-infected mice and the interventional functions of berberine. Methods C57BL/6J mice were divided randomly into 6 groups: Berl group, Ber2 group, azithromycin group, atorvastain group, cholesterol + Cpn group, and normal group. Mice were inoculated intranasally with Cpn for three times, every 2 weeks over a 6-week period and feeding with high cholesterol diet. Berl group was administrated with berberine at the beginning of CPn infecting, while ber2 group, azithromycin group and atorvastain group were administrated with the drug after the 3rd CPn infecting. The serum index, HE dye of the aortas and electron microscopic examination were observed. Results Lipids disorder, increased level of OX-LDL and vascular intimal injury were found. The level ofTC, LDL, TG OX-LDL in Bet group, azithromycin group, and atorvastain group declined in different extent. Conclusion Berberine has effects of regulating lipids disorder, OX-LDL and protecting vascular intima of diet-induced hyperlipidemic and chronic Cpn-infected mice.