国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
4期
309-312
,共4页
郭长青%嵇波%钟鼎文%陈幼楠%金燕%刘清国%郭孟玮%张义%刘乃刚%陈占禄%朱汉章
郭長青%嵇波%鐘鼎文%陳幼楠%金燕%劉清國%郭孟瑋%張義%劉迺剛%陳佔祿%硃漢章
곽장청%혜파%종정문%진유남%금연%류청국%곽맹위%장의%류내강%진점록%주한장
膝骨关节炎%针刀松解法%电针%P物质
膝骨關節炎%針刀鬆解法%電針%P物質
슬골관절염%침도송해법%전침%P물질
Knee osteoarthritis%Acupotomy lysis%Electro-acupunctere%SP
目的 观察针刀松解法对膝骨关节炎(KOA)模型大鼠脊髓及脊髓以上水平P物质(SP)的调节作用,探讨针刀松解法的中枢镇痛机制.方法 将60只健康SD大鼠按照随机数字表分为空白组、KOA模型组、电针组和针刀组.将4%木瓜蛋白酶溶液与0.3 mol/L半胱氨酸溶液按1∶1混合静置0.5 h后分别于造模第1、4、7 d注射于后三组大鼠左膝关节腔,20 μl/次.4周后相应给予针刀松解法治疗或电针治疗.治疗3周后取大鼠腰膨大处脊髓及脑组织,放免法测定脊髓、中脑、垂体、丘脑、下丘脑的SP含量.结果 与空白组比较,KOA模型组的SP含量在脊髓水平(29.094±6.854)pg/mg、脊髓以上水平丘脑(31.043±8.744)pg/mg、下丘脑(75.158±19.546)pg/mg均显著增加(P＜O.05,P＜0.01);电针组的SP含量在脊髓、中脑、丘脑和下丘脑均显著增加(P＜0.05,P＜0.01);针刀组的SP含量在脊髓(29.925±5.286)pg/mg、垂体(55.806±11.152)pg/mg、丘脑(26.500±5.511)pg/mg水平均显著增加(P＜0.05,P＜0.01),在中脑和下丘脑则未见增加.与KOA模型组相比,电针组脊髓SP含量(36.912±6.548)pg/mg显著升高(P＜0.05),电针组和针刀组下丘脑SP含量[分别为:(57.487±14.164)pg/mg、(45.362±6.048)pg/mg]显著降低(P＜0.05,P＜0.01).在下丘脑针刀组较电针组含量低(P＜0.05),在其他部位电针组和针刀组比较,差异无统计学意义.结论 针刀松解法对膝骨关节炎模型大鼠中枢的SP水平有良性调节作用.
目的 觀察針刀鬆解法對膝骨關節炎(KOA)模型大鼠脊髓及脊髓以上水平P物質(SP)的調節作用,探討針刀鬆解法的中樞鎮痛機製.方法 將60隻健康SD大鼠按照隨機數字錶分為空白組、KOA模型組、電針組和針刀組.將4%木瓜蛋白酶溶液與0.3 mol/L半胱氨痠溶液按1∶1混閤靜置0.5 h後分彆于造模第1、4、7 d註射于後三組大鼠左膝關節腔,20 μl/次.4週後相應給予針刀鬆解法治療或電針治療.治療3週後取大鼠腰膨大處脊髓及腦組織,放免法測定脊髓、中腦、垂體、丘腦、下丘腦的SP含量.結果 與空白組比較,KOA模型組的SP含量在脊髓水平(29.094±6.854)pg/mg、脊髓以上水平丘腦(31.043±8.744)pg/mg、下丘腦(75.158±19.546)pg/mg均顯著增加(P＜O.05,P＜0.01);電針組的SP含量在脊髓、中腦、丘腦和下丘腦均顯著增加(P＜0.05,P＜0.01);針刀組的SP含量在脊髓(29.925±5.286)pg/mg、垂體(55.806±11.152)pg/mg、丘腦(26.500±5.511)pg/mg水平均顯著增加(P＜0.05,P＜0.01),在中腦和下丘腦則未見增加.與KOA模型組相比,電針組脊髓SP含量(36.912±6.548)pg/mg顯著升高(P＜0.05),電針組和針刀組下丘腦SP含量[分彆為:(57.487±14.164)pg/mg、(45.362±6.048)pg/mg]顯著降低(P＜0.05,P＜0.01).在下丘腦針刀組較電針組含量低(P＜0.05),在其他部位電針組和針刀組比較,差異無統計學意義.結論 針刀鬆解法對膝骨關節炎模型大鼠中樞的SP水平有良性調節作用.
목적 관찰침도송해법대슬골관절염(KOA)모형대서척수급척수이상수평P물질(SP)적조절작용,탐토침도송해법적중추진통궤제.방법 장60지건강SD대서안조수궤수자표분위공백조、KOA모형조、전침조화침도조.장4%목과단백매용액여0.3 mol/L반광안산용액안1∶1혼합정치0.5 h후분별우조모제1、4、7 d주사우후삼조대서좌슬관절강,20 μl/차.4주후상응급여침도송해법치료혹전침치료.치료3주후취대서요팽대처척수급뇌조직,방면법측정척수、중뇌、수체、구뇌、하구뇌적SP함량.결과 여공백조비교,KOA모형조적SP함량재척수수평(29.094±6.854)pg/mg、척수이상수평구뇌(31.043±8.744)pg/mg、하구뇌(75.158±19.546)pg/mg균현저증가(P＜O.05,P＜0.01);전침조적SP함량재척수、중뇌、구뇌화하구뇌균현저증가(P＜0.05,P＜0.01);침도조적SP함량재척수(29.925±5.286)pg/mg、수체(55.806±11.152)pg/mg、구뇌(26.500±5.511)pg/mg수평균현저증가(P＜0.05,P＜0.01),재중뇌화하구뇌칙미견증가.여KOA모형조상비,전침조척수SP함량(36.912±6.548)pg/mg현저승고(P＜0.05),전침조화침도조하구뇌SP함량[분별위:(57.487±14.164)pg/mg、(45.362±6.048)pg/mg]현저강저(P＜0.05,P＜0.01).재하구뇌침도조교전침조함량저(P＜0.05),재기타부위전침조화침도조비교,차이무통계학의의.결론 침도송해법대슬골관절염모형대서중추적SP수평유량성조절작용.
Objective To observe the regulatory effect of acupotomy lysis on SP level in spinal cord and tissues above spinal cord of rats with knee osteoarthritis.Methods 60 healthy SD rats were randomly divided into normal control group,model group,electro-acupuncture(EA)group,and acupotomy lysis(AL)group.Mix 4%papain solution with 0.3 mol/L cysteine solution in the ratio of 1∶1.After pausing for 0.5h,inject the mixture,20 μl each time,into the left knee joint cavities of rats in model,AL,and EA groups on the day of 1,4,7.After 4 weeks AL group was treated with acupotomy lysis and EA group with electro-acupuncture.Three weeks after treatment,take samples of spinal cord,midbrain,pituitary gland,thalamus,and hypothalamus from the swellings of rats'waists.Measure the content of SP therein separately.Results Compared with normal control group,there was a significant rise in the content of SP in spinal cord and the tissues above spinal cord of model group rats (P＜0.05,P＜0.01),and there was a significant rise in spinal cord,pituitary gland,thalamus and hypothalamus of EA group rats (P＜0.05,P＜0.01);in AL group,there was a significant rise in spinal cord,pituitary gland,thalamus,and there was no statistically difference in hypothalamus and midbrain.Compared with normal control group,there was a significant rise in spinal cord (P＜0.05)and a significant decrease in the SP contents in hypothalamus(P＜0.05,P＜0.01)in EA group.There was no statistically difference between EA group and AL group except in hypothalamus(P＜0.05).Conclusion Acupotomy lysis has positive functions in regulating SP content in centrum of rats with knee osteoarthritis,which helps easing pain.
