CAJ | 학술논문

目的探讨载脂蛋白B(ApoB)基因-516位基因多态性与女性乳腺癌发生的关系.方法运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测200例乳腺癌患者(实验组)和200例正常女性(对照组)的ApoB 基因-516位C/T 基因型和等位基因的分布,并进行对比分析及相关性研究.结果乳腺癌患者中CC、CT基因型的频率分别是78.00％、22.00％,而对照组中则分别为91.50％、8.50％；乳腺癌患者中C、T等位基因的频率分别为89.00％、11.00％,对照组中则分别为95.75％、4.25％,差异均有统计学意义(P＜0.05).CT型个体罹患乳腺癌的风险显著高于CC 型个体(P＜0.05).调整年龄、体质量指数后,携带CT 型的个体较CC 型罹患乳腺癌的风险增加(P＜0.05).结论 ApoB 基因-516位基因多态性与乳腺癌的易感性相关.
목적 탐토재지단백B(ApoB)기인-516위기인다태성여녀성유선암발생적관계.방법 운용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법검측200례유선암환자(실험조)화200례정상녀성(대조조)적ApoB 기인-516위C/T 기인형화등위기인적분포,병진행대비분석급상관성연구.결과 유선암환자중CC、CT기인형적빈솔분별시78.00％、22.00％,이대조조중칙분별위91.50％、8.50％；유선암환자중C、T등위기인적빈솔분별위89.00％、11.00％,대조조중칙분별위95.75％、4.25％,차이균유통계학의의(P＜0.05).CT형개체리환유선암적풍험현저고우CC 형개체(P＜0.05).조정년령、체질량지수후,휴대CT 형적개체교CC 형리환유선암적풍험증가(P＜0.05).결론 ApoB 기인-516위기인다태성여유선암적역감성상관.
Objective To investigate the association of the apolipoprotein B (ApoB) gene-516 C/T polymorphisms with breast cancer in Chinese female population.Methods APoB-516C/T polymorphisms was genotyped by polymerase chain reaction (PCR) and restriction fragment-length polymorphism ( RFLP )in 200 breast cancer patients and 200 controls.The contrastive analysis and the relevant analysis of genotype and allele distribution was performed.Results The frequency of CC and CT genotype in breast cancer group and control group was 78.00％,22.00％,and 91.50％,8.50％,respectively.The frequency of C and T allele in breast cancer group and control group was 89.00％,11.00％,and 95.75％,4.25％,respectively.The risk of breast cancer in the individuals with 516C/T polymorphism was significantly increased.After adjusted for age,and body mass index (BMI) by multiple logistic regression analysis,the odds ratio of T allele carriers for breast cancer was increased as compared with that of non carriers ( OR =3.036; 95％ CI=1.668-5.527 ).Conclusion The ApoB gene-516C/T polymorphism is associated with breast cancer in Chinese female population.