中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
24期
1698-1701
,共4页
吕志栋%徐惠绵%王海波%孔滨%李建国%李福年%宋咏梅
呂誌棟%徐惠綿%王海波%孔濱%李建國%李福年%宋詠梅
려지동%서혜면%왕해파%공빈%리건국%리복년%송영매
胃肿瘤%转化生长因子β1%纤维化%间皮细胞
胃腫瘤%轉化生長因子β1%纖維化%間皮細胞
위종류%전화생장인자β1%섬유화%간피세포
Stomach neoplasms%Transforming growth factor beta1%Fibrosis%Mesothelial cells
目的 观察转化生长因子-β1( TGF-β1)在腹膜纤维化中的作用及其对胃癌细胞黏附的影响.方法TGF-β1作用于人腹膜间皮细胞系HMrSV5,采用RT-PCR、Western印迹、免疫荧光检测纤维黏连蛋白、胶原ⅢmRNA及蛋白水平的表达情况,采用黏附试验观察腹膜纤维化对胃癌细胞系HGC-27、HSC-39黏附的影响.结果(1)5 ng/ml TGF-β1作用间皮细胞24、48、72 h诱导纤维黏连蛋白、胶原ⅢmRNA及蛋白表达,并且呈时间依赖性增加,差异均有统计学意义(均P＜0.01).(2) HGC-27胃癌细胞对TGF-β1作用24、72 h间皮细胞较对照组黏附增加率为65％±5％、124％±11％,差异均有统计学意义(均P＜0.05)；HSC-39胃癌细胞系对TGF-β1作用24、72 h的间皮细胞较对照组黏附增加率为85％±9％、146％±17％,差异均有统计学意义(均P＜0.05).(3) RGD多肽特异性阻断癌细胞与细胞外基质结合的区域后胃癌细胞系HGC-27的黏附数目低于TGF-β1组,黏附减低率为65％±8％,差异有统计学意义(P＜0.05).结论TGF-β1能够诱导间皮细胞纤维黏连蛋白、胶原Ⅲ表达,纤维化的腹膜组织增强胃癌细胞的黏附能力,为癌细胞黏附定植提供适宜的“土壤”环境.
目的 觀察轉化生長因子-β1( TGF-β1)在腹膜纖維化中的作用及其對胃癌細胞黏附的影響.方法TGF-β1作用于人腹膜間皮細胞繫HMrSV5,採用RT-PCR、Western印跡、免疫熒光檢測纖維黏連蛋白、膠原ⅢmRNA及蛋白水平的錶達情況,採用黏附試驗觀察腹膜纖維化對胃癌細胞繫HGC-27、HSC-39黏附的影響.結果(1)5 ng/ml TGF-β1作用間皮細胞24、48、72 h誘導纖維黏連蛋白、膠原ⅢmRNA及蛋白錶達,併且呈時間依賴性增加,差異均有統計學意義(均P＜0.01).(2) HGC-27胃癌細胞對TGF-β1作用24、72 h間皮細胞較對照組黏附增加率為65％±5％、124％±11％,差異均有統計學意義(均P＜0.05)；HSC-39胃癌細胞繫對TGF-β1作用24、72 h的間皮細胞較對照組黏附增加率為85％±9％、146％±17％,差異均有統計學意義(均P＜0.05).(3) RGD多肽特異性阻斷癌細胞與細胞外基質結閤的區域後胃癌細胞繫HGC-27的黏附數目低于TGF-β1組,黏附減低率為65％±8％,差異有統計學意義(P＜0.05).結論TGF-β1能夠誘導間皮細胞纖維黏連蛋白、膠原Ⅲ錶達,纖維化的腹膜組織增彊胃癌細胞的黏附能力,為癌細胞黏附定植提供適宜的“土壤”環境.
목적 관찰전화생장인자-β1( TGF-β1)재복막섬유화중적작용급기대위암세포점부적영향.방법TGF-β1작용우인복막간피세포계HMrSV5,채용RT-PCR、Western인적、면역형광검측섬유점련단백、효원ⅢmRNA급단백수평적표체정황,채용점부시험관찰복막섬유화대위암세포계HGC-27、HSC-39점부적영향.결과(1)5 ng/ml TGF-β1작용간피세포24、48、72 h유도섬유점련단백、효원ⅢmRNA급단백표체,병차정시간의뢰성증가,차이균유통계학의의(균P＜0.01).(2) HGC-27위암세포대TGF-β1작용24、72 h간피세포교대조조점부증가솔위65％±5％、124％±11％,차이균유통계학의의(균P＜0.05)；HSC-39위암세포계대TGF-β1작용24、72 h적간피세포교대조조점부증가솔위85％±9％、146％±17％,차이균유통계학의의(균P＜0.05).(3) RGD다태특이성조단암세포여세포외기질결합적구역후위암세포계HGC-27적점부수목저우TGF-β1조,점부감저솔위65％±8％,차이유통계학의의(P＜0.05).결론TGF-β1능구유도간피세포섬유점련단백、효원Ⅲ표체,섬유화적복막조직증강위암세포적점부능력,위암세포점부정식제공괄의적“토양”배경.
Objective To elucidate the effect of transforming growth factor-beta 1 (TGF-β1) on peritoneal fibrosis and the regulation of gastric cancer adhering to mesothelial cells. Methods The peritoneal mesothelial cell line of HMrSV5 was used to determine the role of TGF-β1 in the regulation of gastric cancer cell adhering to mesothelial cells.And the mRNA and protein expressions of collagen Ⅲ and fibronectin were detected by adhesion assay,Western blot,immunofluorescent staining and reverse transcription-polymerase chain reaction (RT-PCR).Results ( 1 ) The treatment of 5 ng/ml TGF-β1 could induce the expressions of collagen Ⅲ and fibronectin in mesothelial cells at 24,48 and 72 h ( P ＜ 0.01 ).(2) As compared with the controls,the percentages of adhered HGC-27 and HSC-39 gastric cancer cells significantly increased under the treatment of TGF-β1 for 24 and 72 h.The increased adhesion percentages of HGC-27 were 65％ ± 5 ％ and 124％ ± 11％ ( P ＜ 0.05 ) while those of HSC-39 85 ％ ± 9％ and 146％ ± 17％ respectively (P ＜ 0.05 ).( 3 ) Arginylglycyl-aspartic acid (RGD) ( knockdown of minimal sites for cell-binding domain of extracellular matrix) decreased the number of cancer cells adhering to mesothelial cells under the stimulation of TGF-β1.And the decreased adhesion percentage of HGC-27 was 65％ ± 8％ (P ＜ 0.05).Conclusions TGF-β1 significantly stimulates the expressions of collagen Ⅲ and fibronectin in mesothelial cells.And it is associated with the increased adhesion of gastric cancer cell and offers a favorable environment for the dissemination of gastric cancer.
