CAJ | 학술논문

目的多数前列腺癌有两个以上病灶,试图探索这些不同病灶是同源还是各自独立起源.方法选用19例有两个以上病灶的前列腺癌标本,应用显微切割技术获取肿瘤组织,提取DNA.进行PCR扩增,检测染色体8p(D8S133、D8S136、D8S137)和17q(D17S855)4个位点的等位基因杂合性缺失(LOH).结果在19例前列腺癌中发现40个病灶,其D8S133,D8S136,D8S137和D17S855 LOH 率分别为74%、38%、86%和46%.18例中有15例(83%)多发灶LOH不相同,显示其病灶各为独立起源.3例组织学结构显著不同的多发癌灶,LOH为单克隆性(同一来源).结论多数前列腺癌同一肿瘤中不同病灶的起源不同.
목적 다수전렬선암유량개이상병조,시도탐색저사불동병조시동원환시각자독립기원.방법 선용19례유량개이상병조적전렬선암표본,응용현미절할기술획취종류조직,제취DNA.진행PCR확증,검측염색체8p(D8S133、D8S136、D8S137)화17q(D17S855)4개위점적등위기인잡합성결실(LOH).결과 재19례전렬선암중발현40개병조,기D8S133,D8S136,D8S137화D17S855 LOH 솔분별위74%、38%、86%화46%.18례중유15례(83%)다발조LOH불상동,현시기병조각위독립기원.3례조직학결구현저불동적다발암조,LOH위단극륭성(동일래원).결론 다수전렬선암동일종류중불동병조적기원불동.
Objecfive Most prostate cancer contains two or more widely separate turnors.To study the origin of prostate cancer based on the analysis of microsatellite alteration in separate tumors from the same prostate.Methods A polymerase chain reaction (PCR) was used to examine the allelic loss pattern of 4 microsatellite polymorphic markers on chromosome 8p (D8S133,D8S136,D8S137) and 17q (D17S855) in multifocal tumors of prostate from 19 patients.DNA samples were obtained from different regions of distinctly separate tumors on single case using microdissection technique.Results The overall frequence of LOH at D8S133,D8S136,D8S137 and D17S855 for all informative cases was 74%,38%,86% and 46%respectively in 40 separate tumors of prostate from 19 patients.The pattern of allelic loss was not identical in 15 of 18 (83%) informative cases. It showed that the different regions of prostate cancer were independent origin respectively.Discordant pattern of histology was observe in distantly separate regions.whereas the same allele was consistently lost in samples from different regions of the same tumor in 3 cases. Condusion Current data supports independent origin of multiple tumors in most prostate cancer patients.