CAJ | 학술논문

目的:探讨新生隐球菌荚膜体外形成条件,为实验教学提供一种简便、安全、成本低、效果好的实验方法.方法:采用试管培养法,用麦芽糖代替葡萄糖配制培养基并加入不同浓度的兔血清和(或)维生素B1,观察新生隐球菌在不同培养基上荚膜的形成情况.结果:新生隐球菌在含1.4mL 6%麦芽糖+0.4mL兔血清+0.2mL维生素B1的培养基中培养4天荚膜形成最佳.结论:在1.4 mL 6%麦芽糖+0.4 mL兔血清+0.2 mL维生素B1的培养基中新生隐球菌可形成典型荚膜.
목적:탐토신생은구균협막체외형성조건,위실험교학제공일충간편、안전、성본저、효과호적실험방법.방법:채용시관배양법,용맥아당대체포도당배제배양기병가입불동농도적토혈청화(혹)유생소B1,관찰신생은구균재불동배양기상협막적형성정황.결과:신생은구균재함1.4mL 6%맥아당+0.4mL토혈청+0.2mL유생소B1적배양기중배양4천협막형성최가.결론:재1.4 mL 6%맥아당+0.4 mL토혈청+0.2 mL유생소B1적배양기중신생은구균가형성전형협막.
Objective:To research the condition of the capsule in-vitro forming of Cryptococcus neoformans,providing a simple,safe,low-cost,elective experimental method for experimental teaching.Method:With the method of tube cultivation,maltose which take the place of the glucose was used to make the culture medium,rabbit's serum and (or) Vitamin B1 was added to the culture medium,to observe the forming of the capsule of Cryptococeus neoformans in different culture medium.Result:The capsule of Cryptococcus neoformans grew best in the culture medium which made up of 1.4 mL 6% maltose + 0.4 mL rabbit's serum +0.2 mL Vitamin B1 cultivated for four days.Conclusions:Cryptococcus neoformans can form typical capsule in the culture medium which made up of 1.4 mL 6% maltose +0.4 mL rabbit's serum +0.2 mL Vitamin B1.