福建农林大学学报（自然科学版）
福建農林大學學報（自然科學版）
복건농림대학학보（자연과학판）
JOURNAL OF FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2010年
1期
30-36
,共7页
黄花青花菜%茎段%离体培养%快繁
黃花青花菜%莖段%離體培養%快繁
황화청화채%경단%리체배양%쾌번
yellow-flower broccoli%stem cuttings%in vitro culture%micropropagation
以黄花青花菜杂种1代无菌试管苗茎段为材料,进行增殖、生根、移栽等试验.结果表明:茎段增殖的适宜培养基为MS+0.2 mg·L(-1) BA+0.01 mg·L~(-1) NAA,增殖系数为2.95,苗质量等级为1.67;诱导生根的适宜培养基为1/2MS+0.2 mg·L~(-1) IBA,生根率为94.45%,苗质量等级为3.00;长势良好的植株,株高4-5 cm,根数4-5条,根长2-3 cm,移栽至泥炭土:蛭石:河沙=1:1:1的混合基质中,成活率达83.33%.
以黃花青花菜雜種1代無菌試管苗莖段為材料,進行增殖、生根、移栽等試驗.結果錶明:莖段增殖的適宜培養基為MS+0.2 mg·L(-1) BA+0.01 mg·L~(-1) NAA,增殖繫數為2.95,苗質量等級為1.67;誘導生根的適宜培養基為1/2MS+0.2 mg·L~(-1) IBA,生根率為94.45%,苗質量等級為3.00;長勢良好的植株,株高4-5 cm,根數4-5條,根長2-3 cm,移栽至泥炭土:蛭石:河沙=1:1:1的混閤基質中,成活率達83.33%.
이황화청화채잡충1대무균시관묘경단위재료,진행증식、생근、이재등시험.결과표명:경단증식적괄의배양기위MS+0.2 mg·L(-1) BA+0.01 mg·L~(-1) NAA,증식계수위2.95,묘질량등급위1.67;유도생근적괄의배양기위1/2MS+0.2 mg·L~(-1) IBA,생근솔위94.45%,묘질량등급위3.00;장세량호적식주,주고4-5 cm,근수4-5조,근장2-3 cm,이재지니탄토:질석:하사=1:1:1적혼합기질중,성활솔체83.33%.
The in vitro cuttings of F1 hybrid of yellow-flower broccoli (Brassica oleracea L. var. italica Phenck) were used as the materials for the studies of proliferation, rooting, and transplanting of micropropagation in broccoli. The results showed that the best medium for proliferation was MS with 0. 2 mg·L~(-1) BA and 0. 01 mg·L~(-1) NAA， the mean coefficient of proliferation was 2. 95, the quality of seedling grade was 1. 67; the appropriate rooting induction medium was 1/2MS with 0. 2 mg·L~(-1) IBA, the rooting rate reached 94. 45%, and the quality of seedling grade was 3. 00. In the well-growth plantlets, the average plantlet height was between 4 and 5 cm, the average root number was between 4 and 5, and the average root length was between 2 and 3 cm. When the plantlets were transferred into the mixed media (peaty soil, vermiculite and sand), and the survival rate was up to 83. 33%.
