CAJ | 학술논문

背景:目前国内外已有关于人脐血间充质干细胞移植修复鼠脊髓损伤、脑肿瘤、心肌梗死等的报道,将其在特定的条件下向成骨细胞诱导分化的研究也已有报道,但尚未有将脐血间充质干细胞移植治疗动物骨坏死的研究报道.目的:观察重组增强型绿色荧光蛋白慢病毒载体示踪转染的脐血间充质干细胞移植修复兔股骨头缺血性坏死的效果.方法:含骨形态发生蛋白2基因质粒与携带重组增强型绿色荧光蛋白的慢病毒载体与脐血间充质干细胞共培养;制作兔股骨头缺损模型,随机分为3组,正常组未作任何处理,对照组骨缺损未进行填充;实验组骨缺损填充重组增强型绿色荧光蛋白慢病毒载体示踪转染的脐血间充质干细胞;分别于治疗4周和8周时股骨头行影像学和组织学观察.结果与结论:影像学和组织学检查显示实验组治疗4周时即有明显的成骨反应和新骨形成,8周时基本修复股骨头的骨缺损区;对照组治疗4周时骨缺损为纤维结缔组织填充,8周时股骨头缺损周边骨质硬化,骨缺损处充填纤维结缔组织,股骨头骨小梁紊乱.结果显示重组增强型绿色荧光蛋白慢病毒载体示踪转染的脐血间充质干细胞有较强的诱导成骨作用,可以成功的修复股骨头缺损性坏死.
배경:목전국내외이유관우인제혈간충질간세포이식수복서척수손상、뇌종류、심기경사등적보도,장기재특정적조건하향성골세포유도분화적연구야이유보도,단상미유장제혈간충질간세포이식치료동물골배사적연구보도.목적:관찰중조증강형록색형광단백만병독재체시종전염적제혈간충질간세포이식수복토고골두결혈성배사적효과.방법:함골형태발생단백2기인질립여휴대중조증강형록색형광단백적만병독재체여제혈간충질간세포공배양;제작토고골두결손모형,수궤분위3조,정상조미작임하처리,대조조골결손미진행전충;실험조골결손전충중조증강형록색형광단백만병독재체시종전염적제혈간충질간세포;분별우치료4주화8주시고골두행영상학화조직학관찰.결과여결론:영상학화조직학검사현시실험조치료4주시즉유명현적성골반응화신골형성,8주시기본수복고골두적골결손구;대조조치료4주시골결손위섬유결체조직전충,8주시고골두결손주변골질경화,골결손처충전섬유결체조직,고골두골소량문란.결과현시중조증강형록색형광단백만병독재체시종전염적제혈간충질간세포유교강적유도성골작용,가이성공적수복고골두결손성배사.
BACKGROUND: At present, studies concerning human umbilical cord blood mesenchymal stem cells (UCB-MSCs) transplantation for repair of rat spinal cord injury, brain tumor, and myocardial infarction have been reported, and studies that human UCB-MSCs were induced differentiation into osteogenic cells under certain conditions have also been reported at home and abroad. But application of UCB-MSCs transplantation in the treatment of osteonecrosis of animals has not yet been reported. OBJECTIVE: To observe the repair results of recombinant lentivirus vector tracing enhanced green fluorescent protein (EGFP)-transfected UCB-MSCs transplantation in treatment of ischemic necrosis of the femoral head in rabbits. METHODS: Bone morphogenetic protein-2 gene plasmid, recombinant lentivirus vector carrying EGFP and UCB-MSCs were co-cultured. Rabbit models of femoral head defects were made and randomly divided into 3 groups. There was no treatment in the normal group, control group with bone defects and experimental bone defects filled with UCB-MSCs tracing transfected by recombinant lentivirus vector carrying EGFP. At 4 and 8 weeks after treatment, the imaging and histological of the femoral head were observed.RESULTS AND CONCLUSION: Imaging and histology results showed that there were osteogenic response and new bone formation in the experimental group at 4 weeks, and the bone defects were basically repaired at 8 weeks after treatment. In the control group, the bone defects filled with fibrous connective tissue fiber connective tissues at 4 weeks, and the osteosclerosis could be found surrounding femoral head, bone defects filled with fibrous connective tissue fibers and bone trabecula distributed disorderly. The recombinant lentivirus vector tracing EGFP-transfected into UCB-MSCs has strong effects bone conduction and can repair ischemic necrosis of the femoral head.