CAJ | 학술논문

目的研究let-7b对人黑色素瘤细胞增殖及有氧糖酵解的影响.方法将人工合成的microRNA单核苷酸let-7b mimics及inhibitor通过脂质体转染人黑色素瘤细胞株A375,上调或抑制let-7b在细胞内的表达水平,不同时相点检测细胞葡萄糖消耗和乳酸产量.MTT法检测细胞增殖.运用单因素方差分析探讨let-7b表达水平与黑色素瘤细胞有氧糖酵解及增殖的关系.结果 let-7bmimics组24、48 h时相点检测MTT吸光度值分别为0.396±0.030、0.525±0.037,低于其他组(P＜0.05),提示高表达let-7b抑制细胞增殖；各组黑色素瘤细胞24、48 h葡萄糖消耗及乳酸产量差异无统计学意义(P＞0.05),乳酸产量与葡萄糖消耗比值各组间差异无统计学意义(P＞0.05),其中空白组在24h和48 h内葡萄糖转化为乳酸效率约为57％和43％.结论黑色素瘤A375细胞具有显著的有氧糖酵解表型,过表达let-7b可抑制人恶性黑色素瘤细胞A375增殖,但对于细胞的有氧糖酵解效率,尚未发现let-7b对其有显著影响.
목적 연구let-7b대인흑색소류세포증식급유양당효해적영향.방법 장인공합성적microRNA단핵감산let-7b mimics급inhibitor통과지질체전염인흑색소류세포주A375,상조혹억제let-7b재세포내적표체수평,불동시상점검측세포포도당소모화유산산량.MTT법검측세포증식.운용단인소방차분석탐토let-7b표체수평여흑색소류세포유양당효해급증식적관계.결과 let-7bmimics조24、48 h시상점검측MTT흡광도치분별위0.396±0.030、0.525±0.037,저우기타조(P＜0.05),제시고표체let-7b억제세포증식；각조흑색소류세포24、48 h포도당소모급유산산량차이무통계학의의(P＞0.05),유산산량여포도당소모비치각조간차이무통계학의의(P＞0.05),기중공백조재24h화48 h내포도당전화위유산효솔약위57％화43％.결론 흑색소류A375세포구유현저적유양당효해표형,과표체let-7b가억제인악성흑색소류세포A375증식,단대우세포적유양당효해효솔,상미발현let-7b대기유현저영향.
Objective To study the influence of let-7b on cell proliferation and aerobic glycolysis of human melanoma cell A375.Methods Transfect A375 cell line with hsa-let-7b oligonucleotide or antisense.Glucose and lactate in medium were determined by spectrophotometry at 24 h and 48 h time point after transfection.The cell proliferation was determined by methylthiazol tetrazolium (MTT) assay.Results Over expression of let-7b in melanoma cell reduced cell proliferation notably,compared to the other groups by MTT(P ＜0.05).However,the glucose consumption and lactate production differences were not observed during 24 h or 48 h ( P ＞ 0.05 ),the blank control group transformed about 57％ and 43％ glucose to lactate during 24 h and 48 h.Conclusions Melanoma cell line A375 has notably aerobic glycolysis hallmark,let-7b could inhibit proliferation of melanoma cell line A375,but it may has no influence on glucose metabolism.