中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
3期
312-315
,共4页
陈荣%沈定霞%闫中强%王欢
陳榮%瀋定霞%閆中彊%王歡
진영%침정하%염중강%왕환
金黄色葡萄球菌%血流感染%抗药性%基因分型%分子流行病学
金黃色葡萄毬菌%血流感染%抗藥性%基因分型%分子流行病學
금황색포도구균%혈류감염%항약성%기인분형%분자류행병학
Staphylococcus aureus%Bloodstream infection%Drug-resistance%Genotyping%Molecular epidemiology
目的 研究金黄色葡萄球菌临床血流感染分离株的分子流行病学特征.方法 收集2006年1月至2008年12月解放军总医院分离的临床血流感染金黄色葡萄球菌菌株(共47株),标本来源均为患者静脉血.采用琼脂稀释法检测所有菌株对多种抗生素的耐药性,PCR方法 检测pvl毒素基因,DiversiLab~(TM)Rep-PCR分型系统分析菌株的同源性;对耐甲氧西林金黄色葡萄球菌(MRSA)进行葡萄球菌染色体/mec(SCCmec)分型以及ST239型别的快速筛查;综合分型和药敏试验结果,挑选部分代表菌株进行多位点序列分型(MLST).结果 47株血流感染金黄色葡萄球菌中,pvl基因检出率为4.3%.MRSA占51.1%.MRSA均为SCCmec Ⅲ型菌株;Rep-PCR分为A～L共12个型,其中A型为最主要的型,共22株(46.8%),所有的MRSA均属于A、B两型.结论 47株临床血流感染金黄色葡萄球菌中的MRSA绝大部分为多重耐药克隆ST239-MRSA-SCCmecⅢ型.
目的 研究金黃色葡萄毬菌臨床血流感染分離株的分子流行病學特徵.方法 收集2006年1月至2008年12月解放軍總醫院分離的臨床血流感染金黃色葡萄毬菌菌株(共47株),標本來源均為患者靜脈血.採用瓊脂稀釋法檢測所有菌株對多種抗生素的耐藥性,PCR方法 檢測pvl毒素基因,DiversiLab~(TM)Rep-PCR分型繫統分析菌株的同源性;對耐甲氧西林金黃色葡萄毬菌(MRSA)進行葡萄毬菌染色體/mec(SCCmec)分型以及ST239型彆的快速篩查;綜閤分型和藥敏試驗結果,挑選部分代錶菌株進行多位點序列分型(MLST).結果 47株血流感染金黃色葡萄毬菌中,pvl基因檢齣率為4.3%.MRSA佔51.1%.MRSA均為SCCmec Ⅲ型菌株;Rep-PCR分為A～L共12箇型,其中A型為最主要的型,共22株(46.8%),所有的MRSA均屬于A、B兩型.結論 47株臨床血流感染金黃色葡萄毬菌中的MRSA絕大部分為多重耐藥剋隆ST239-MRSA-SCCmecⅢ型.
목적 연구금황색포도구균림상혈류감염분리주적분자류행병학특정.방법 수집2006년1월지2008년12월해방군총의원분리적림상혈류감염금황색포도구균균주(공47주),표본래원균위환자정맥혈.채용경지희석법검측소유균주대다충항생소적내약성,PCR방법 검측pvl독소기인,DiversiLab~(TM)Rep-PCR분형계통분석균주적동원성;대내갑양서림금황색포도구균(MRSA)진행포도구균염색체/mec(SCCmec)분형이급ST239형별적쾌속사사;종합분형화약민시험결과,도선부분대표균주진행다위점서렬분형(MLST).결과 47주혈류감염금황색포도구균중,pvl기인검출솔위4.3%.MRSA점51.1%.MRSA균위SCCmec Ⅲ형균주;Rep-PCR분위A～L공12개형,기중A형위최주요적형,공22주(46.8%),소유적MRSA균속우A、B량형.결론 47주림상혈류감염금황색포도구균중적MRSA절대부분위다중내약극륭ST239-MRSA-SCCmecⅢ형.
Objective To investigate the molecular epidemiological characteristics of Staphylococcus aureus associated with bloodstream infection in hospital. Methods 47 Staphylococcus aureus strains isolated from bloodstream in PLA General Hospital were collected from January 2006 to December 2008. Susceptibility of the strains to 11 antimicrobial agents was detected and DNA homology of them was analyzed with Rep-based DiversiLab~(TM) Microbial Typing System. Panton-Valentine leukocidin (PVL)gene was determined by PCR. For methicillin-resistant Staphylococcus aureus (MRSA) strains,the genotypes of SCCmec were determined and ST239 clone was screened with multiplex PCR. Multilocus sequence typing (MLST) was used to determine the STs of the selected isolates. Results In the 47 Staphylococcus aureus isolated from blood,methicillin-resistant strains accounted for 51.1%,all belonged to SCCmec Ⅲ type,with only 2 pvl gene positive strains identified. 12 different patterns (A-L) were found among 47 strains with Rep-PCR. All MRSA strains clustered in the A and B subtypes. Conclusion Most MRSA strains isolated from blood in PLA General Hospital belonged to ST239-MRSA-SCCmec Ⅲ clone. DiversiLab~(TM) Microbial Typing System could provide a rapid and effective method to investigate the molecular epidemiological characteristics of Staphylococcus aureus in the hospital settings.
