中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
1期
10-13
,共4页
王海萍%张乃红%邢冬红%汤晓菲%王兆娥%黄焕军%白虹
王海萍%張迺紅%邢鼕紅%湯曉菲%王兆娥%黃煥軍%白虹
왕해평%장내홍%형동홍%탕효비%왕조아%황환군%백홍
沙眼衣原体鼠肺炎株%IFN-γ%Th17
沙眼衣原體鼠肺炎株%IFN-γ%Th17
사안의원체서폐염주%IFN-γ%Th17
Chlamydia muridarum%IFN-γ%Th17
目的 探讨IFN-γ在小鼠沙眼衣原体感染中对Th17/IL-17应答的调节作用.方法 利用沙眼衣原体鼠肺炎株小鼠呼吸道感染模型,用抗鼠IFN-γ单克隆抗体吸入中和肺组织IFN-γ,对照组给予同等剂量的独特型抗体IgG2a,于感染后7 d处死小鼠.免疫酶法检测小鼠肺组织衣原体生长;利用RT-PCR技术检测衣原体感染小鼠肺组织中Th17相关因子IL-17及其上游因子IL-23 mRNA的表达;细胞内细胞因子染色检测衣原体感染小鼠脾脏IL-17-CD4+T细胞的扩增.结果 与对照组相比,IFN-γ抗体中和小鼠有严重的疾病状态,包括明显的体重下降、肺组织更高的衣原体负荷和肺组织更严重的病理损伤;肺组织IL-17和IL-23 mRNA的表达水平显著降低;脾脏IL-17-CD4+T细胞百分率也显著降低.结论 小鼠衣原体感染中,IFN-γ通过上调Th17/IL-17应答起保护作用.
目的 探討IFN-γ在小鼠沙眼衣原體感染中對Th17/IL-17應答的調節作用.方法 利用沙眼衣原體鼠肺炎株小鼠呼吸道感染模型,用抗鼠IFN-γ單剋隆抗體吸入中和肺組織IFN-γ,對照組給予同等劑量的獨特型抗體IgG2a,于感染後7 d處死小鼠.免疫酶法檢測小鼠肺組織衣原體生長;利用RT-PCR技術檢測衣原體感染小鼠肺組織中Th17相關因子IL-17及其上遊因子IL-23 mRNA的錶達;細胞內細胞因子染色檢測衣原體感染小鼠脾髒IL-17-CD4+T細胞的擴增.結果 與對照組相比,IFN-γ抗體中和小鼠有嚴重的疾病狀態,包括明顯的體重下降、肺組織更高的衣原體負荷和肺組織更嚴重的病理損傷;肺組織IL-17和IL-23 mRNA的錶達水平顯著降低;脾髒IL-17-CD4+T細胞百分率也顯著降低.結論 小鼠衣原體感染中,IFN-γ通過上調Th17/IL-17應答起保護作用.
목적 탐토IFN-γ재소서사안의원체감염중대Th17/IL-17응답적조절작용.방법 이용사안의원체서폐염주소서호흡도감염모형,용항서IFN-γ단극륭항체흡입중화폐조직IFN-γ,대조조급여동등제량적독특형항체IgG2a,우감염후7 d처사소서.면역매법검측소서폐조직의원체생장;이용RT-PCR기술검측의원체감염소서폐조직중Th17상관인자IL-17급기상유인자IL-23 mRNA적표체;세포내세포인자염색검측의원체감염소서비장IL-17-CD4+T세포적확증.결과 여대조조상비,IFN-γ항체중화소서유엄중적질병상태,포괄명현적체중하강、폐조직경고적의원체부하화폐조직경엄중적병리손상;폐조직IL-17화IL-23 mRNA적표체수평현저강저;비장IL-17-CD4+T세포백분솔야현저강저.결론 소서의원체감염중,IFN-γ통과상조Th17/IL-17응답기보호작용.
Objective To investigate the regulation of IFN-γ to Th17 response in Chlamydia muridarum (Cm) lung infection in mice. Methods A murine model of pneumonia induced by intranasal inoculation of Cm was used for this study. Anti-mouse IFN-γ McAbs were used to neutralize endogenous IFN-γfollowing Cm lung infection. Control group received the same dose of isotype antibody (IgG2a). Mice were sacrificed at day 7 postinfection. Chlamydial growth in the lung was assessed by immunoenzyme technique.IL-17 and IL-23 mRNA expression in the lung was assayed by RT-PCR and the proliferation of IL-17 + CD4 +T cells in the spleen was assayed by intracellular cytokine staining. Results IFN-γ-neutralized mice exhibited serious disease course, include greater body weight loss, higher organism growth and much more severe pathological changes in the lung compared with control mice. The mRNA expression of IL-17 and IL-23 in the lung and the proliferation of IL-17 + CD4 + T cells in the spleen significantly decreased in the IL-17- neutralized mice. Conclusion IFN-γ was protective in Cm lung infection through up-regulating the antigen specific Th17 responses.