中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2012年
10期
67-69
,共3页
张田%姚书霞%高俊%申世兰
張田%姚書霞%高俊%申世蘭
장전%요서하%고준%신세란
骨肉瘤%EphA2%CyclinD1
骨肉瘤%EphA2%CyclinD1
골육류%EphA2%CyclinD1
Osteosarcoma%EphA2%CyclinD1
目的 探讨CyclinD1和EphA2在骨肉瘤组织中的表达和临床意义.方法 应用免疫组织化学SP法分别对50例骨肉瘤组织、20例骨软骨瘤组织及20例正常骨组织中EphA2和CyclinDl蛋白的表达进行检测并对结果进行统计学分析.结果 正常骨组织、骨软骨瘤组织CyclinDl和EphA2蛋白阳性表达率为0(0/20),骨肉瘤组织中CyclinD1和EphA2蛋白阳性表达率分别为70.00％(35/50)和78.00％ (39/50),差异均有统计学意义(P＜0.05).I级、Ⅱ级和Ⅲ级骨肉瘤组织中EphA2和CyclinD1蛋白阳性表达率在不同临床分期之间两两相比差异有统计学意义(P＜0.05).伴有肺转移骨肉瘤组织中EphA2和CyclinDl蛋白阳性表达率与无肺转移骨肉瘤组织表达率相比,差异有统计学意义(P＜0.05).EphA2和CyclinD1蛋白在骨肉瘤组织中的表达,经统计学分析,呈明显正相关(P＜0.01).结论 EphA2和CyclinDl蛋白表达与骨肉瘤的演进有关,EphA2和CyclinD1蛋白在骨肉瘤组织中的表达有相关性.同时,二者可能在骨肉瘤的发生、发展及浸润、转移过程中起协同作用.
目的 探討CyclinD1和EphA2在骨肉瘤組織中的錶達和臨床意義.方法 應用免疫組織化學SP法分彆對50例骨肉瘤組織、20例骨軟骨瘤組織及20例正常骨組織中EphA2和CyclinDl蛋白的錶達進行檢測併對結果進行統計學分析.結果 正常骨組織、骨軟骨瘤組織CyclinDl和EphA2蛋白暘性錶達率為0(0/20),骨肉瘤組織中CyclinD1和EphA2蛋白暘性錶達率分彆為70.00％(35/50)和78.00％ (39/50),差異均有統計學意義(P＜0.05).I級、Ⅱ級和Ⅲ級骨肉瘤組織中EphA2和CyclinD1蛋白暘性錶達率在不同臨床分期之間兩兩相比差異有統計學意義(P＜0.05).伴有肺轉移骨肉瘤組織中EphA2和CyclinDl蛋白暘性錶達率與無肺轉移骨肉瘤組織錶達率相比,差異有統計學意義(P＜0.05).EphA2和CyclinD1蛋白在骨肉瘤組織中的錶達,經統計學分析,呈明顯正相關(P＜0.01).結論 EphA2和CyclinDl蛋白錶達與骨肉瘤的縯進有關,EphA2和CyclinD1蛋白在骨肉瘤組織中的錶達有相關性.同時,二者可能在骨肉瘤的髮生、髮展及浸潤、轉移過程中起協同作用.
목적 탐토CyclinD1화EphA2재골육류조직중적표체화림상의의.방법 응용면역조직화학SP법분별대50례골육류조직、20례골연골류조직급20례정상골조직중EphA2화CyclinDl단백적표체진행검측병대결과진행통계학분석.결과 정상골조직、골연골류조직CyclinDl화EphA2단백양성표체솔위0(0/20),골육류조직중CyclinD1화EphA2단백양성표체솔분별위70.00％(35/50)화78.00％ (39/50),차이균유통계학의의(P＜0.05).I급、Ⅱ급화Ⅲ급골육류조직중EphA2화CyclinD1단백양성표체솔재불동림상분기지간량량상비차이유통계학의의(P＜0.05).반유폐전이골육류조직중EphA2화CyclinDl단백양성표체솔여무폐전이골육류조직표체솔상비,차이유통계학의의(P＜0.05).EphA2화CyclinD1단백재골육류조직중적표체,경통계학분석,정명현정상관(P＜0.01).결론 EphA2화CyclinDl단백표체여골육류적연진유관,EphA2화CyclinD1단백재골육류조직중적표체유상관성.동시,이자가능재골육류적발생、발전급침윤、전이과정중기협동작용.
Objective To study the expression and clinical significance of EphA2 and CyclinD1 in osteosarcoma tissue.Methods The expressions of EphA2 and CyclinD1 in 50 cases of osteosarcoma tissues,20 cases of osteochondroma tissues and 20 cases of normal bone tissues were deteted by immunohisto-chemistry SP method,and the results were analyzed statistically.Results The positive expression rates of EphA2 and CyclinD1 proteins in normal bone and osteochondroma tissues were 0(0/20) ; The positive expression rates of EphA2 and CyclinD 1 proteins in osteosarcoma tissues were 78.00％ (39/50)and 70％ (35/50),respectively.There were significant differences( P ＜0.05).The positive expression rates of EphA2 and CyclinD1 proteins in osteosarcoma tissues with grade Ⅲ were higher than those in grade Iand grade Ⅱ.There were significant differences between different clinical stages (P ＜ 0.05 ).There were significant differences in the positive expression rates of EphA2 and CyclinDl proteins in osteesarcoma tissues with and without metastasis (P ＜ 0.05).There was positive correlation of the EphA2 expression with CyclinD1 expression in osteosarcoma tissues (P ＜ 0.01).Conclusions The expressions of EphA2 and CyclinD1 proteins are related with progression of osteosarcoma.There is positive correlation between the expressions of EphA2 and CyclinD1 proteins in osteosarcoma tissues.EphA2 and CyclinDl protein can play synergistic effect on the process of occurrence,development and infiltration,metastasis of osteosarcoma.
