CAJ | 학술논문

通过选择性富集培养,从济南市南部山区所取的土样中分离得到 1 株联苯降解菌,实验证明该菌株能以联苯作为惟一的碳源和能源生长,初步命名为LB07,经形态学、生理生化鉴定和16S rRNA基因序列对比分析,确定该菌属于巨大芽胞杆菌.分别研究pH、温度、底物浓度、装液量和培养时间各个单因素对联苯降解率的影响.然后设计正交实验确定菌株降解联苯的最佳优化条件,最佳培养条件为联苯初始质量浓度 150 mg/L,温度 35 ℃,pH=6.5,装液量为 250 mL三角瓶装 50 mL,培养时间 10 d,菌株LB07降解联苯的性能最好,降解率达到94%.
통과선택성부집배양,종제남시남부산구소취적토양중분리득도 1 주련분강해균,실험증명해균주능이련분작위유일적탄원화능원생장,초보명명위LB07,경형태학、생리생화감정화16S rRNA기인서렬대비분석,학정해균속우거대아포간균.분별연구pH、온도、저물농도、장액량화배양시간각개단인소대련분강해솔적영향.연후설계정교실험학정균주강해련분적최가우화조건,최가배양조건위련분초시질량농도 150 mg/L,온도 35 ℃,pH=6.5,장액량위 250 mL삼각병장 50 mL,배양시간 10 d,균주LB07강해련분적성능최호,강해솔체도94%.
A biphenyl-degrading bacterial strain was isolated from soil in mountain area of southern Jinan by selective enrichment culture. The strain, named as LB07, was able to use biphenyl as an unique carbon and energy source for growth. It belongs Bacillus megaterium based on the data about morphological, physio-biochemical identification and 16S rRNA sequence analysis. The factors that affecting the strain's biphenyl degrading ability such as pH, temperature, initial biphenyl concentration, volume of medium, inoculating dosage and reaction time were determined. The optimal conditions for the biphenyl degradation were definited through orthogonal test. The results showed that the biodegrading rate could reach the highest as 94% under the conditions of 150 mg/L substrate concentration, 35 ℃ incubation temperature, pH 6.5, 50 mL/250 mL medium volume and 10 d reaction time.