CAJ | 학술논문

通过PCR方法,将麻疯树核糖体失活蛋白curcin的开放阅读框片段从麻疯树总DNA中扩增出来.并将该克隆基因片段连接到原核表达载体pQE-30中,成功构建了重组质粒pQE-C,转化大肠杆菌M15菌株.经IPTG诱导后,curcin重组蛋白在大肠杆菌中成功表达.然后通过纯化原核表达产物,得到纯化的curcin重组蛋白,并以纸片法进行体外抗菌活性检测,发现其具有较强抗真菌活性.
통과PCR방법,장마풍수핵당체실활단백curcin적개방열독광편단종마풍수총DNA중확증출래.병장해극륭기인편단련접도원핵표체재체pQE-30중,성공구건료중조질립pQE-C,전화대장간균M15균주.경IPTG유도후,curcin중조단백재대장간균중성공표체.연후통과순화원핵표체산물,득도순화적curcin중조단백,병이지편법진행체외항균활성검측,발현기구유교강항진균활성.
The open reading frame (ORF)of curcin was amplified from total DNA of Jatropha curcas by PCR method. The cloned gene fragments were ligated into a prokaryotic expression vector pQE-30 and the recombination plasmid pQE-C was constructed. Then the pQE-C was transformed into E. coli M15, and the recombination curcin was expressed in M15 under IPTG induction. The purified recombination curcin was obtained and its antibacterial activity was tested in vitro by paper disk method. It was found that curcin had a better antifungal activity.