CAJ | 학술논문

目的研究标本放置时间对部分血凝检测结果的影响,为规范分析前过程提供实验依据.方法随机选取100份枸橼酸钠抗凝外周血标本分别在室温放置1、2、4、6、8和10 h后进行血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)和纤维蛋白原(Fbg)含量的测定.根据不同检测项目和时间段分组,使用SAS统计软件包对上述4项指标在放置2、4、6、8、10 h所得到的检测结果与放置1 h内检测的结果进行配对t检验.结果 PT、TT测定在标本采集1 h内的结果与2和4 h后检测结果差异无统计学意义(P>0.05),与6 h后检测的结果之间差异有统计学意义(P<0.01).APTT测定在标本采集1 h内检测的结果与2、4、6、8和10 h后检测的结果之间差异有统计学意义(P<0.01).Fbg测定在标本采集1 h内的结果与2 h检测的结果差异无统计学意义(P>0.05),而与之后的结果差异有统计学意义(P<0.01).结论用于凝血试验的标本在室温存放一段时间后,对不同检测项目均造成一定影响,标本采集后应在1 h内检测,如不能做到也应在2 h内检测完毕,否则会对检测结果造成明显影响.
목적 연구표본방치시간대부분혈응검측결과적영향,위규범분석전과정제공실험의거.방법 수궤선취100빈구연산납항응외주혈표본분별재실온방치1、2、4、6、8화10 h후진행혈장응혈매원시간(PT)、활화부분응혈활매시간(APTT)、응혈매시간(TT)화섬유단백원(Fbg)함량적측정.근거불동검측항목화시간단분조,사용SAS통계연건포대상술4항지표재방치2、4、6、8、10 h소득도적검측결과여방치1 h내검측적결과진행배대t검험.결과 PT、TT측정재표본채집1 h내적결과여2화4 h후검측결과차이무통계학의의(P>0.05),여6 h후검측적결과지간차이유통계학의의(P<0.01).APTT측정재표본채집1 h내검측적결과여2、4、6、8화10 h후검측적결과지간차이유통계학의의(P<0.01).Fbg측정재표본채집1 h내적결과여2 h검측적결과차이무통계학의의(P>0.05),이여지후적결과차이유통계학의의(P<0.01).결론 용우응혈시험적표본재실온존방일단시간후,대불동검측항목균조성일정영향,표본채집후응재1 h내검측,여불능주도야응재2 h내검측완필,부칙회대검측결과조성명현영향.
Objective To investigate the influence of sample storage time on results of blood coagulation test in room temperature in order to provide experimental reference in regulating the pre-analysis procedure. Methods 100 blood samples were selected randomly and stored in room temperature for 2 hours [prothrombin time (PT) 2, activated partial thromboplastin time (APTT) 2, thrombin time (TT) 2 and fibrinogen (Fbg) 2], 4 hours (PT4, APTT4, TT4, Fbg4), 6 hours (PT6, APTT6, TT6, Fbg6), 8 hours (PT8, APTT8, TT8, Fbg8) and 10 hours (PT10, APTT10, TT10, Fbg10) respectively before the determinations of PT, APTT, TT and Fbg while the results were compared with those of less than 1 hour storage (PT1, APTT1, TT1, Fbg1). The results were classified by different test items and time, using Statistics Analysis System (SAS) software to do statistical analysis (paired t test). Results There was no statistical difference between PT1 and PT2, PT4 (P>0.05). There was no statistical difference between TT1 and TT2, TT4 (P>0.05). There was statistical difference between PT1, PT6 and TT1, TT6 (P<0.01). There was statistical difference between APTT1 and APTT2, APTT4, APTT6, APTT8 and APTT10 (P<0.01). There was no statistical difference between Fbg1 and Fbg2 (P>0.05). There was statistical difference between Fbg1 and Fbg4, Fbg6, Fbg8 and Fbg10 (P<0.01). Conclusions Samples which were collected for coagulation test should be determined immediately and without delay to avoid reduction in their activities. If the coagulation test can not be determined within 2 hours, there will be significant influence on the results.