CAJ | 학술논문

利用FreeBSD操作系统的文本过滤命令将NCBI的Genbank数据库的EST序列实现本地化导入到MySQL数据库中并能够进行更新,这利于对不同物种不同组织器官基因表达的分析.以水稻EST数据为例,对EST序列两端出现的polyA/T和载体序列亦进行了鉴别及去除,经过预处理的EST序列数据将为进一步进行EST聚类及基因表达分析提供可靠的保证.
이용FreeBSD조작계통적문본과려명령장NCBI적Genbank수거고적EST서렬실현본지화도입도MySQL수거고중병능구진행경신,저리우대불동물충불동조직기관기인표체적분석.이수도EST수거위례,대EST서렬량단출현적polyA/T화재체서렬역진행료감별급거제,경과예처리적EST서렬수거장위진일보진행EST취류급기인표체분석제공가고적보증.
EST sequences in Genbank of NCBI were stored locally in MySQL database extracted by FreeBSD text filtering commands,which can be updated from NCBI and will be useful for analysing EST like in different tissues from a special species. Rice EST were analysed by identifying and removal of polyA/T and sequences of cloning vectors in flanking regions of EST,which will provide better guarantee for further EST clustering and gene expression analysis.