化学分析计量
化學分析計量
화학분석계량
CHEMICAL ANALYSIS AND METERAGE
2010年
3期
42-44
,共3页
关托拉宗%牛血清白蛋白%荧光光谱
關託拉宗%牛血清白蛋白%熒光光譜
관탁랍종%우혈청백단백%형광광보
metolazone%bovine serum albumin%fluorescence spectroscopy
采用荧光光谱技术研究了在pH7．40的Tris—HCl缓冲介质中关托拉宗与BSA相互作用。实验发现，美托拉宗对BSA有较强的荧光猝灭作用，根据292K和311K时关托拉宗对BSA的荧光猝灭作用，利用Stern—Volmer方程及双倒数方程处理实验数据，表明美托拉宗对BSA的荧光猝灭作用属于动态猝灭过程，根据Forster非辐射能量转移理论计算出了美托拉宗与BSA间的结合距离r=2．77nm，结合过程的热力学数据表明，二者主要靠疏水作用力结合；进一步采用同步荧光光谱探讨了美托拉宗对BSA构象的影响。
採用熒光光譜技術研究瞭在pH7．40的Tris—HCl緩遲介質中關託拉宗與BSA相互作用。實驗髮現，美託拉宗對BSA有較彊的熒光猝滅作用，根據292K和311K時關託拉宗對BSA的熒光猝滅作用，利用Stern—Volmer方程及雙倒數方程處理實驗數據，錶明美託拉宗對BSA的熒光猝滅作用屬于動態猝滅過程，根據Forster非輻射能量轉移理論計算齣瞭美託拉宗與BSA間的結閤距離r=2．77nm，結閤過程的熱力學數據錶明，二者主要靠疏水作用力結閤；進一步採用同步熒光光譜探討瞭美託拉宗對BSA構象的影響。
채용형광광보기술연구료재pH7．40적Tris—HCl완충개질중관탁랍종여BSA상호작용。실험발현，미탁랍종대BSA유교강적형광졸멸작용，근거292K화311K시관탁랍종대BSA적형광졸멸작용，이용Stern—Volmer방정급쌍도수방정처리실험수거，표명미탁랍종대BSA적형광졸멸작용속우동태졸멸과정，근거Forster비복사능량전이이론계산출료미탁랍종여BSA간적결합거리r=2．77nm，결합과정적열역학수거표명，이자주요고소수작용력결합；진일보채용동보형광광보탐토료미탁랍종대BSA구상적영향。
The binding reaction of metolazone with bovine serum albumin was studied at 292K and 311K by fluorescence quenching spectra and synchronous fluorescence spectra. It was shown that metolazone had a strong ability of quenching the fluorescence of BSA. The Stern - Volmer curve and double logarithm equation of the fluorescence quenching of BSA by metolazone indicated that the quenching mechanism of metolazone to BSA was a dynamic quenching. According to the Forster＇ s theory of non - radiation energy transfer, the binding distances（r） was 2.77 nm （292K）. The thermodynamic parameters showed that the interaction of metolazone and BSA was mainly driven by hydrophobic force. Synchronous spectrum was used to investigate the conformational changes of BSA.
