中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2010年
6期
511-512
,共2页
呼吸道合胞病毒%毛细支气管炎%白细胞介素-10%基因多态性
呼吸道閤胞病毒%毛細支氣管炎%白細胞介素-10%基因多態性
호흡도합포병독%모세지기관염%백세포개소-10%기인다태성
Respiratory syncytial virus%Bronchiolitis%Interleukin-10%Gene polymorphism
目的 探讨白细胞介素-10-592A/C基因多态性与呼吸道合胞病毒(RSV)毛细支气管炎易感性、病情的关联和对血清白细胞介素(IL)-10的影响.方法 采用聚合酶链反应-限制性片段长度多态性检测100例汉族RSV毛细支气管炎患儿(病例组)和100例健康儿童(对照组)IL-10-592A/C位点单核苷酸多态性;应用ELISA法检测病例组血清IL-10水平.结果 病例组IL-10-592A/C位点基因型频率分别为AA 44%、AC 38%、CC 18%,等位基因频率分别为A 63%、C 37%;对照组基因型频率分别为AA 41%、AC 42%、CC 17%,等位基因频率分别为A 64%、C 36%;两组基因型及等位基因频率比较差异均无显著性(χ2=0.33,P>0.05;χ2=0.43,P>0.05).病例组IL-10-592A/C位点不同基因型患儿血清IL-10水平比较差异无显著性(F=0.87,P>0.05).IL-10-592A/C位点基因型频率在轻度和中重度患儿之间的差异无显著性(χ2=2.67,P>0.05).结论 IL-10-592A/C位点基因多态性与RSV毛细支气管炎不存在关联.
目的 探討白細胞介素-10-592A/C基因多態性與呼吸道閤胞病毒(RSV)毛細支氣管炎易感性、病情的關聯和對血清白細胞介素(IL)-10的影響.方法 採用聚閤酶鏈反應-限製性片段長度多態性檢測100例漢族RSV毛細支氣管炎患兒(病例組)和100例健康兒童(對照組)IL-10-592A/C位點單覈苷痠多態性;應用ELISA法檢測病例組血清IL-10水平.結果 病例組IL-10-592A/C位點基因型頻率分彆為AA 44%、AC 38%、CC 18%,等位基因頻率分彆為A 63%、C 37%;對照組基因型頻率分彆為AA 41%、AC 42%、CC 17%,等位基因頻率分彆為A 64%、C 36%;兩組基因型及等位基因頻率比較差異均無顯著性(χ2=0.33,P>0.05;χ2=0.43,P>0.05).病例組IL-10-592A/C位點不同基因型患兒血清IL-10水平比較差異無顯著性(F=0.87,P>0.05).IL-10-592A/C位點基因型頻率在輕度和中重度患兒之間的差異無顯著性(χ2=2.67,P>0.05).結論 IL-10-592A/C位點基因多態性與RSV毛細支氣管炎不存在關聯.
목적 탐토백세포개소-10-592A/C기인다태성여호흡도합포병독(RSV)모세지기관염역감성、병정적관련화대혈청백세포개소(IL)-10적영향.방법 채용취합매련반응-한제성편단장도다태성검측100례한족RSV모세지기관염환인(병례조)화100례건강인동(대조조)IL-10-592A/C위점단핵감산다태성;응용ELISA법검측병례조혈청IL-10수평.결과 병례조IL-10-592A/C위점기인형빈솔분별위AA 44%、AC 38%、CC 18%,등위기인빈솔분별위A 63%、C 37%;대조조기인형빈솔분별위AA 41%、AC 42%、CC 17%,등위기인빈솔분별위A 64%、C 36%;량조기인형급등위기인빈솔비교차이균무현저성(χ2=0.33,P>0.05;χ2=0.43,P>0.05).병례조IL-10-592A/C위점불동기인형환인혈청IL-10수평비교차이무현저성(F=0.87,P>0.05).IL-10-592A/C위점기인형빈솔재경도화중중도환인지간적차이무현저성(χ2=2.67,P>0.05).결론 IL-10-592A/C위점기인다태성여RSV모세지기관염불존재관련.
Objective To explore the association of IL-10-592A/C polymorphism with the susceptibility to respiratory syncytial virus(RSV) bronchiolitis and disease severity,and to explore the impact of IL10-592A/C polymorphism upon total serum IL-10 levels in children with RSV bronchiolitis. Methods One hundred children (patients group) admitted to hospital with RSV bronchiolitis and 100 healthy children (control group) were recruited. PCR restriction length fragment polymorphism assay was used to detect the single nucleotide polymorphisms of IL-10-592A/C. Total serum IL-10 levels in patients group were assayed with ELISA. Results The genotype frequency of IL-10-592A/C were AA 44% ,AC 38% ,CC 18% in patients group ,and AA 41% ,AC 42% ,CC 17% in the control group,it showed no significant difference between two groups (χ2= 0. 33, P > 0. 05) . The frequency of each allele were A 63%, C 37 % in patient group, and A 64% ,C 36% in the control group,there was no significant difference in allele frequency between two groups (χ2=0. 43 ,P >0. 05) . There was no significant difference in total serum IL-10 levels between different genotype of IL-10-592A/C (F = 0. 87, P > 0. 05) . The differences in genotype frequency of IL-10-592A/C were not significant between mild and moderate to severe cases (χ2= 2. 67, P > 0. 05) . Conclusion IL-10-592A/C gene polymorphisms do not correlated with RSV bronchiolitis.
