中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2011年
1期
40-43
,共4页
刘娣%许晓群%陈雪梅%张建华%陈志鸿%于启海%王郡甫
劉娣%許曉群%陳雪梅%張建華%陳誌鴻%于啟海%王郡甫
류제%허효군%진설매%장건화%진지홍%우계해%왕군보
肾母细胞瘤%转化生长因子β%逆转录聚合酶链反应
腎母細胞瘤%轉化生長因子β%逆轉錄聚閤酶鏈反應
신모세포류%전화생장인자β%역전록취합매련반응
Nephroblastoma%Transforming growth factor beta%Reverse transcriptase polymerase chain reaction
目的 探讨叉头样转录因子3(FOXP3)及转化生长因子β(TGF-β)mRNA在小儿肾母细胞瘤中的表达和临床意义.方法 采用RT-PCR方法检测30例肾母细胞瘤组织、30例瘤旁组织和3例正常肾组织中FOXP3及TGF-βmRNA的表达情况,观察两者表达与临床分期的关系.结果 与瘤旁组织和正常组织比较,FOXP3及TGF-βmRNA在肿瘤组织中表达均上升,为0.706±0.107、0.667±0.121,差异具有统计学意义(P＜0.001);二者mRNA在Ⅰ期肿瘤中的表达强度为0.535±0.086、0.564±0.050均低于Ⅱ期的0.741±0.074、0.704±0.067和Ⅲ期的0.843±0.053、0.734±0.085,差异具有统计学意义(P＜0.001);FOXP3和TGF-βmRNA表达强度呈明显正相关(r=0.749,P＜0.001).结论 小儿肾母细胞瘤组织中,FOXP3及TGF-βmRNA表达增高,可能与肾母细胞瘤的发展和转移有关.
目的 探討扠頭樣轉錄因子3(FOXP3)及轉化生長因子β(TGF-β)mRNA在小兒腎母細胞瘤中的錶達和臨床意義.方法 採用RT-PCR方法檢測30例腎母細胞瘤組織、30例瘤徬組織和3例正常腎組織中FOXP3及TGF-βmRNA的錶達情況,觀察兩者錶達與臨床分期的關繫.結果 與瘤徬組織和正常組織比較,FOXP3及TGF-βmRNA在腫瘤組織中錶達均上升,為0.706±0.107、0.667±0.121,差異具有統計學意義(P＜0.001);二者mRNA在Ⅰ期腫瘤中的錶達彊度為0.535±0.086、0.564±0.050均低于Ⅱ期的0.741±0.074、0.704±0.067和Ⅲ期的0.843±0.053、0.734±0.085,差異具有統計學意義(P＜0.001);FOXP3和TGF-βmRNA錶達彊度呈明顯正相關(r=0.749,P＜0.001).結論 小兒腎母細胞瘤組織中,FOXP3及TGF-βmRNA錶達增高,可能與腎母細胞瘤的髮展和轉移有關.
목적 탐토차두양전록인자3(FOXP3)급전화생장인자β(TGF-β)mRNA재소인신모세포류중적표체화림상의의.방법 채용RT-PCR방법검측30례신모세포류조직、30례류방조직화3례정상신조직중FOXP3급TGF-βmRNA적표체정황,관찰량자표체여림상분기적관계.결과 여류방조직화정상조직비교,FOXP3급TGF-βmRNA재종류조직중표체균상승,위0.706±0.107、0.667±0.121,차이구유통계학의의(P＜0.001);이자mRNA재Ⅰ기종류중적표체강도위0.535±0.086、0.564±0.050균저우Ⅱ기적0.741±0.074、0.704±0.067화Ⅲ기적0.843±0.053、0.734±0.085,차이구유통계학의의(P＜0.001);FOXP3화TGF-βmRNA표체강도정명현정상관(r=0.749,P＜0.001).결론 소인신모세포류조직중,FOXP3급TGF-βmRNA표체증고,가능여신모세포류적발전화전이유관.
Objective To investigate the expression levels and the clinical significance of FOXP3and TGF-β in nephroblastoma. Methods The expression levels of FOXP3 and TGF-β were evaluated using RT-RCR in 30 nephroblastoma,30 adjacent tissues and 3 normal tissues. Results The expression levels of FOXP3 and TGF-β in nephroblastoma tissues were higher than adjacent and normal tissues(P＜0. 001). Compared to the stage Ⅱ and Ⅲ the difference of the expression levels of FOXP3and TGF-β in stage Ⅰ were significant in nephroblastoma tissues(P＜0. 001). There was a positive correlation between FOXP3 and TGF-β. Conclusions The expression levels of FOXP3 and TGF-β were higher in tumor tisssues, suggesting that it may be related to metastasis and progression of the nephroblastoma.
