中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2010年
6期
736-739
,共4页
陈雄%孙维佳%廖洁%段小辉%陆晔斌
陳雄%孫維佳%廖潔%段小輝%陸曄斌
진웅%손유가%료길%단소휘%륙엽빈
抗坏血酸/投药和剂量%胰腺肿瘤/病理学/代谢%肿瘤侵润%基质金属蛋白酶2/代谢/药物作用
抗壞血痠/投藥和劑量%胰腺腫瘤/病理學/代謝%腫瘤侵潤%基質金屬蛋白酶2/代謝/藥物作用
항배혈산/투약화제량%이선종류/병이학/대사%종류침윤%기질금속단백매2/대사/약물작용
Ascorbic acid/AD%Pancreatic neoplasms/PA/ME%Neoplasm invasiveness%Matrix metalloproteinase 2/ME/DE
目的 探讨大剂量维生素C对胰腺癌细胞株PANC-1细胞基质金属蛋白酶2(MMP2)的表达和侵袭能力的影响,为临床维生素C治疗胰腺癌可行性提供理论依据.方法 采用transwell侵袭实验比较不同浓度维生素C组PANC-1细胞的侵袭能力,并运用RT-PCR和Western b1ot检测并比较各组细胞MMP2 mRNA和蛋白水平的表达情况.结果 1.0、5.0和10.0 mM浓度维生素C处理组MMP2 mRNA的相对丰度值较空白对照组显著降低(0.510±0.004 vs 0.792±0.006,0.391±0.007 vs 0.792±0.006,0.282±0.008 vs 0.792±0.006,P＜0.05).1.0、5.0和10.0 mM浓度维生素C处理组MMP2蛋白的相对丰度值较空白对照组显著降低(0.519±0.004 vs 0.761±0.014,0.310±0.007 vs 0.761±0.014,0.297±0.008 vs 0.761±0.014,P＜0.05).1.0、5.0和10.0 mM浓度维生素C处理组穿过transwell的细胞数相较空白对照组显著降低(452±22 vs 653±28,340±32 vs 653±28,409±33 vs 653±28,P＜0.05).结论 大剂量维生素C可以降低PANC-1细胞MMP2的表达,并可减弱其侵袭能力.
目的 探討大劑量維生素C對胰腺癌細胞株PANC-1細胞基質金屬蛋白酶2(MMP2)的錶達和侵襲能力的影響,為臨床維生素C治療胰腺癌可行性提供理論依據.方法 採用transwell侵襲實驗比較不同濃度維生素C組PANC-1細胞的侵襲能力,併運用RT-PCR和Western b1ot檢測併比較各組細胞MMP2 mRNA和蛋白水平的錶達情況.結果 1.0、5.0和10.0 mM濃度維生素C處理組MMP2 mRNA的相對豐度值較空白對照組顯著降低(0.510±0.004 vs 0.792±0.006,0.391±0.007 vs 0.792±0.006,0.282±0.008 vs 0.792±0.006,P＜0.05).1.0、5.0和10.0 mM濃度維生素C處理組MMP2蛋白的相對豐度值較空白對照組顯著降低(0.519±0.004 vs 0.761±0.014,0.310±0.007 vs 0.761±0.014,0.297±0.008 vs 0.761±0.014,P＜0.05).1.0、5.0和10.0 mM濃度維生素C處理組穿過transwell的細胞數相較空白對照組顯著降低(452±22 vs 653±28,340±32 vs 653±28,409±33 vs 653±28,P＜0.05).結論 大劑量維生素C可以降低PANC-1細胞MMP2的錶達,併可減弱其侵襲能力.
목적 탐토대제량유생소C대이선암세포주PANC-1세포기질금속단백매2(MMP2)적표체화침습능력적영향,위림상유생소C치료이선암가행성제공이론의거.방법 채용transwell침습실험비교불동농도유생소C조PANC-1세포적침습능력,병운용RT-PCR화Western b1ot검측병비교각조세포MMP2 mRNA화단백수평적표체정황.결과 1.0、5.0화10.0 mM농도유생소C처리조MMP2 mRNA적상대봉도치교공백대조조현저강저(0.510±0.004 vs 0.792±0.006,0.391±0.007 vs 0.792±0.006,0.282±0.008 vs 0.792±0.006,P＜0.05).1.0、5.0화10.0 mM농도유생소C처리조MMP2단백적상대봉도치교공백대조조현저강저(0.519±0.004 vs 0.761±0.014,0.310±0.007 vs 0.761±0.014,0.297±0.008 vs 0.761±0.014,P＜0.05).1.0、5.0화10.0 mM농도유생소C처리조천과transwell적세포수상교공백대조조현저강저(452±22 vs 653±28,340±32 vs 653±28,409±33 vs 653±28,P＜0.05).결론 대제량유생소C가이강저PANC-1세포MMP2적표체,병가감약기침습능력.
Objective To observe the effect of high-dose vitamin C on MMP2 expression and invasive ability in PANC-1. Methods Transwell invasion assay was used to compare the invasive ability of PANC-1 cells in different concentrations of vitamin C treated groups. RT-PCR and Western blot were used to detect and compare the levels of MMP2 mRNA and protein expression in each group. Results Compared with the 0mM vitamin C treated group, the mRNA expression of MMP2 was significantly decreased in 1.0,5.0,10. 0mM group(0. 510 ±0. 004 vs 0. 792 ±0. 006, 0. 391 ±0. 007 vs 0. 792 ±0. 006, 0. 282 ±0. 008vs 0. 792 ± 0. 006, P ＜ 0. 05 ). Compared with the 0mM vitamin C treated group, the protein expression of MMP2 was significantly decreased in 1.0,5.0,10. 0mM group(0. 519 ±0. 004 vs 0. 761 ±0. 014,0. 310 ±0. 007 vs 0. 761 ±0. 014,0. 297 ±0. 008 vs 0. 761 ±0. 014, P ＜0. 05). Compared with the 0mM vitamin C treated group, the invaded cell number was significantly decreased in 1.0,5.0,10. 0mM groups ( 452 ± 22 vs653 ± 28,340 ± 32 vs 653 ± 28,409 ± 33 vs 653 ± 28, P ＜ 0. 05 ). Conclusion High-dose vitamin C can decrease the expression of MMP2 in PANC-1 cells, and weaken its invasive ability.
