CAJ | 학술논문

目的研究溶质转运蛋白家族11成员1(SLC11AI)基因单倍型与中国藏族人群肺结核易感性的关系.方法 2004年6月至2005年1月选取藏族肺结核患者140例,感染MTB未发病的藏族对照139例,用变性高效液相色谱检测和PCR限制性片段长度多态性分析方法对SLC11A基因4种多态性[5'(GT)n、INT4、D53N和3'UTR]进行基因分型,用SHESIS软件进行连锁不平衡与单倍型分析,以X2检验研究基因单倍型与肺结核易感性的关系.结果病例组5'(CT)9/INT4 G单倍型频率(181/280,64.8%)显著低于对照组(217/276,78.1%),差异有统计学意义(X2=11.026,P<0.01);病例组3['UTR TGTG/D543N G单倍型频率(215/280,76.6%)显著低于对照组(235/276,84.4%),差异有统计学意义(X2:6.547,P<0.05);3'UTR TGTG缺失/D543N A单倍型频率(34/280,12.0%)显著高于对照组(18/276,6.4%),差异有统计学意义(X2=6.547,P<0.05).结论 SL11AI摹闪中5'(GT)/INT4 G、3'UTR TGTG/D543N G及其3'UTR TGTG缺失/D543N A单倍型与中国藏族人群肺结核易感性有关.
목적 연구용질전운단백가족11성원1(SLC11AI)기인단배형여중국장족인군폐결핵역감성적관계.방법 2004년6월지2005년1월선취장족폐결핵환자140례,감염MTB미발병적장족대조139례,용변성고효액상색보검측화PCR한제성편단장도다태성분석방법대SLC11A기인4충다태성[5'(GT)n、INT4、D53N화3'UTR]진행기인분형,용SHESIS연건진행련쇄불평형여단배형분석,이X2검험연구기인단배형여폐결핵역감성적관계.결과 병례조5'(CT)9/INT4 G단배형빈솔(181/280,64.8%)현저저우대조조(217/276,78.1%),차이유통계학의의(X2=11.026,P<0.01);병례조3['UTR TGTG/D543N G단배형빈솔(215/280,76.6%)현저저우대조조(235/276,84.4%),차이유통계학의의(X2:6.547,P<0.05);3'UTR TGTG결실/D543N A단배형빈솔(34/280,12.0%)현저고우대조조(18/276,6.4%),차이유통계학의의(X2=6.547,P<0.05).결론 SL11AI모섬중5'(GT)/INT4 G、3'UTR TGTG/D543N G급기3'UTR TGTG결실/D543N A단배형여중국장족인군폐결핵역감성유관.
Objective To investigate the association of the haplotype of the solute carrier family 11 member 1 (SLC11AI) gene with susceptibility to pulmonary tuberculosis in Tibetans.Methods Four polymorphisms of the SLC11AI gene [5'(GT)n,INT4,D53N,and 3' UTR]were investigated by denaturalization high performance liquid chromatography and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 140 patients (the patient group) and 139 PPD-positive healthy controls (the control group) of the Tibetan nationality from June 2004 until January 2005.The relationship between the haplotype and susceptibility to pulmonary tuberculosis in these patients was studied by X2 test,and the linkage disequilibrium as well as the haplotype were analyzed by the SHESIS software.Results The haplotype frequencies of 5'(GT)9/INT4 G,3'UTR TGTG/D543N G,3'UTR TGTG del/D543N A were 64.8% (181/280),76.6% (215/280),12.0% (34/280) among the patients and 78.1% (217/276),84.4% (235/276),6.4% (18/276) among the controls.5' (GT)9/INT4 G,3' UTR TGTG/D543N G haplotypes rendered a lower risk (X2 = 11.026,P < 0.01,X2 = 6.547,P < 0.05,respectively),but 3'UTR TGTG del/D543N A haplotype a higher risk (X2 = 6.547,P < 0.05) for tuberculosis.Conclusions 5' (GT)9/INT4 G,3'UTR TGTG/D543N G and 3'UTR TGTG del/D543N A haplotypes of the SLC11AI genemay be associated with the susceptibility of the Tibetan population to pulmonary tuberculosis.