中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2009年
5期
387-391
,共5页
于桂花%李荣山%乔唏%周芸%寇敏%王晨%白波%邵珊
于桂花%李榮山%喬唏%週蕓%寇敏%王晨%白波%邵珊
우계화%리영산%교희%주예%구민%왕신%백파%소산
肾上腺髓质素%受体,降钙素%再灌注损伤%肾小管坏死,急性
腎上腺髓質素%受體,降鈣素%再灌註損傷%腎小管壞死,急性
신상선수질소%수체,강개소%재관주손상%신소관배사,급성
Adrenomedullin%Receptors,calcitonin%Reperfusion injury%Kidneytubular necrosis,acute
目的 观察垂体中叶素(IMD)及其受体降钙素受体样受体(CRLR)在大鼠肾脏缺血再灌注损伤中的表达变化.方法 将健康雄性Wistar大鼠随机分为假手术组和手术组,夹闭大鼠双侧.肾动脉制作肾脏缺血再灌注损伤(IRI)模型,于缺血再灌注后0、6、12、24、48、72 h 6个时间点各取6只大鼠,留取血清及肾组织标本,对肾脏病理损伤评分并行半定是量分析;Western印迹法半定量分析肾组织IMD及其受体CRLR的表达变化;放射免疫法检测血浆中IMD的表达变化.结果 手术组大鼠发生了急性肾小管坏死(ATN),以缺血再灌注48 h时病理损伤最重.与假手术组比较.IMD及CRLR在缺血再灌注12、24、48、72 h表达显著增高(均P<0.01);血浆中IMD在缺血再灌注12、48、72 h表达显著增高(均P<0.05).结论 IMD及受体CRLR在大鼠肾脏缺血再灌注损伤中表达增加,血浆中IMD表达上调,提示其可能在肾脏缺血再灌注损伤病理生理过程中发挥作用.
目的 觀察垂體中葉素(IMD)及其受體降鈣素受體樣受體(CRLR)在大鼠腎髒缺血再灌註損傷中的錶達變化.方法 將健康雄性Wistar大鼠隨機分為假手術組和手術組,夾閉大鼠雙側.腎動脈製作腎髒缺血再灌註損傷(IRI)模型,于缺血再灌註後0、6、12、24、48、72 h 6箇時間點各取6隻大鼠,留取血清及腎組織標本,對腎髒病理損傷評分併行半定是量分析;Western印跡法半定量分析腎組織IMD及其受體CRLR的錶達變化;放射免疫法檢測血漿中IMD的錶達變化.結果 手術組大鼠髮生瞭急性腎小管壞死(ATN),以缺血再灌註48 h時病理損傷最重.與假手術組比較.IMD及CRLR在缺血再灌註12、24、48、72 h錶達顯著增高(均P<0.01);血漿中IMD在缺血再灌註12、48、72 h錶達顯著增高(均P<0.05).結論 IMD及受體CRLR在大鼠腎髒缺血再灌註損傷中錶達增加,血漿中IMD錶達上調,提示其可能在腎髒缺血再灌註損傷病理生理過程中髮揮作用.
목적 관찰수체중협소(IMD)급기수체강개소수체양수체(CRLR)재대서신장결혈재관주손상중적표체변화.방법 장건강웅성Wistar대서수궤분위가수술조화수술조,협폐대서쌍측.신동맥제작신장결혈재관주손상(IRI)모형,우결혈재관주후0、6、12、24、48、72 h 6개시간점각취6지대서,류취혈청급신조직표본,대신장병리손상평분병행반정시량분석;Western인적법반정량분석신조직IMD급기수체CRLR적표체변화;방사면역법검측혈장중IMD적표체변화.결과 수술조대서발생료급성신소관배사(ATN),이결혈재관주48 h시병리손상최중.여가수술조비교.IMD급CRLR재결혈재관주12、24、48、72 h표체현저증고(균P<0.01);혈장중IMD재결혈재관주12、48、72 h표체현저증고(균P<0.05).결론 IMD급수체CRLR재대서신장결혈재관주손상중표체증가,혈장중IMD표체상조,제시기가능재신장결혈재관주손상병리생리과정중발휘작용.
Objective To investigate the expressions of intermedin /adrenomeduliin 2 (IMD/AM2) and its receptor calcitonin receptor-like receptor (CRLR) in the kidney of rats after renal ischemia reperfusion injury(IRI). Methods Male Wistar rats were divided randomly into two groups: sham group and operation group. Renal IRI model was induced by clamping both renal arteries. Blood and kidney were harversted at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h after reperfusion, respectively. Renal histological changes were semi-quantitated. Expressions of IMD and CRLR in the kidney were detected by Western blot, and the content of IMD in serum was measured by radioimmunity at 12 h, 48 h, 72 h after repeffusion. Results Kidneys of renal IRI model rats displayed significant pathologic changes, and the changes were much severer at 48 h after reperfusion. The expressions of IMD and CRLR in kidney were significantly up-regnlated at 12 h, 48 h, 72 h after renal IRI (P<0.01). The level of IMD in serum increased at 12 h, 48 h, 72 h after renal IRI (P<0.05). Conclusion The expressions of IMD and its receptor are up-regulated in the kidney after renal IRI, which may participate in the pathophysiological changes induced by renal IRI.
