中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
25期
1773-1777
,共5页
汪有锦%姚群立%方芳%滕皋军
汪有錦%姚群立%方芳%滕皋軍
왕유금%요군립%방방%등고군
磁共振成像%脑梗塞%缺血半暗带
磁共振成像%腦梗塞%缺血半暗帶
자공진성상%뇌경새%결혈반암대
Magnetic resonance imaging%Brain infarction%Ischemic penumbra
目的 探讨高场强磁共振(7.0T)灌注和弥散加权成像在大鼠急性脑梗死缺血半暗带研究中的价值.方法 利用线栓法建立大鼠急性脑梗死模型.60只SD大鼠随机分成6组,即假手术对照组,栓塞0.5、1.5、3、6和24 h组,每组10只.各组大鼠于相应时间点行头颅MRI扫描:PWI、DWI、T1WI、T2WI及MRA.后处理获得脑血容量(CBV)、脑血流量(CBF)、平均通过时间(MTT)形态图,并分别计算rCBV、rCBF、rMTT相对值.测量DWI异常信号区相对体积(rVD)及PWI异常灌注区相对体积(rVP).将结果与四氮唑红(TTC)染色和病理对比.结果 假手术组各序列扫描、TTC染色及病理均未见异常.各栓塞组栓塞侧大脑中动脉供血区rCBV、rCBF明显降低,rMTT明显延长,梗死核心严重,梗死边缘较轻.各栓塞组栓塞侧大脑中动脉供血区于DWI和PWI均可见异常信号区;DWI异常信号区体积随栓塞时间延长而增大,PWI异常信号区体积随时间延长变化不明显,栓塞6 h前PWI异常信号区大于DWI异常信号区(P<0.05),6 h以后PWI、DWI梗死体积大小差异无统计学意义(P>0.05).3 h以后DWI梗死体积与24 hTTC梗死体积差异无统计学意义(P>0.05).结论 高场强磁共振灌注和弥散加权成像动态显示急性脑梗塞缺血半暗带的时间、空间变化,为进一步研究提供基础.
目的 探討高場彊磁共振(7.0T)灌註和瀰散加權成像在大鼠急性腦梗死缺血半暗帶研究中的價值.方法 利用線栓法建立大鼠急性腦梗死模型.60隻SD大鼠隨機分成6組,即假手術對照組,栓塞0.5、1.5、3、6和24 h組,每組10隻.各組大鼠于相應時間點行頭顱MRI掃描:PWI、DWI、T1WI、T2WI及MRA.後處理穫得腦血容量(CBV)、腦血流量(CBF)、平均通過時間(MTT)形態圖,併分彆計算rCBV、rCBF、rMTT相對值.測量DWI異常信號區相對體積(rVD)及PWI異常灌註區相對體積(rVP).將結果與四氮唑紅(TTC)染色和病理對比.結果 假手術組各序列掃描、TTC染色及病理均未見異常.各栓塞組栓塞側大腦中動脈供血區rCBV、rCBF明顯降低,rMTT明顯延長,梗死覈心嚴重,梗死邊緣較輕.各栓塞組栓塞側大腦中動脈供血區于DWI和PWI均可見異常信號區;DWI異常信號區體積隨栓塞時間延長而增大,PWI異常信號區體積隨時間延長變化不明顯,栓塞6 h前PWI異常信號區大于DWI異常信號區(P<0.05),6 h以後PWI、DWI梗死體積大小差異無統計學意義(P>0.05).3 h以後DWI梗死體積與24 hTTC梗死體積差異無統計學意義(P>0.05).結論 高場彊磁共振灌註和瀰散加權成像動態顯示急性腦梗塞缺血半暗帶的時間、空間變化,為進一步研究提供基礎.
목적 탐토고장강자공진(7.0T)관주화미산가권성상재대서급성뇌경사결혈반암대연구중적개치.방법 이용선전법건립대서급성뇌경사모형.60지SD대서수궤분성6조,즉가수술대조조,전새0.5、1.5、3、6화24 h조,매조10지.각조대서우상응시간점행두로MRI소묘:PWI、DWI、T1WI、T2WI급MRA.후처리획득뇌혈용량(CBV)、뇌혈류량(CBF)、평균통과시간(MTT)형태도,병분별계산rCBV、rCBF、rMTT상대치.측량DWI이상신호구상대체적(rVD)급PWI이상관주구상대체적(rVP).장결과여사담서홍(TTC)염색화병리대비.결과 가수술조각서렬소묘、TTC염색급병리균미견이상.각전새조전새측대뇌중동맥공혈구rCBV、rCBF명현강저,rMTT명현연장,경사핵심엄중,경사변연교경.각전새조전새측대뇌중동맥공혈구우DWI화PWI균가견이상신호구;DWI이상신호구체적수전새시간연장이증대,PWI이상신호구체적수시간연장변화불명현,전새6 h전PWI이상신호구대우DWI이상신호구(P<0.05),6 h이후PWI、DWI경사체적대소차이무통계학의의(P>0.05).3 h이후DWI경사체적여24 hTTC경사체적차이무통계학의의(P>0.05).결론 고장강자공진관주화미산가권성상동태현시급성뇌경새결혈반암대적시간、공간변화,위진일보연구제공기출.
Objective To approach the value of perfusion-weighed and diffusion-weighted imaging at high field intensity (7.0 T) MR in research of the ischemic penumbra in acute cerebral infarction of rats.Methods Acute cerebral infarction of rat was induced by octluding middie cerebral artery (MCAO) with suture.Sixty SD rats were divided into six groups randomly of ten including sham operation group.and groups of MCAO for 0.5,1.5,3.6,24 h.Then all rats were examined by PWI,T1WI,T2WI and MRA at corresponding time point.Regional cerebral blood volume (rCBV),relative cerebral blood flow (rCBF),mean transit time (MTT) topographical maps were reconstructed and the relative values of rCBV,rCBF,MTT,DWI-derived (rVD) and PWI-derived (rVP) lesion volume were calculated.The outcomes of serial MRI were compared with TTC stain and pathological findings.Results Neither abnormal sisnal of MBI nor TTC stain or pathological changes were found in sham operation group.The rCBV.rCBF was significantly decreased but rMTT obviously extended of the area of middle cerebral artery blood-supply of each group of MCAO.The core of infarction was more serious than the edge of infarction. Abnormal signals were found in the MCA-supplying regions in the DWI and PWI of all of the groups of MCAO.The size of rVD became bigger with the time going on but no significant difference of rVP The size of rVP was larger than rVD before MCAO of 6h(P<0.05).There was no significant difference between PWI and DWI after 6 h (P>0.05) and also between TTC at 24 h and DWI after 3 h (P>0.05).Conclusion Perfusion-weighed and diffusion-weighted imaging at high field intensity (7.0 T) MR dynamic displaying the existence and the process of ischemic penumbra in acute cerebral infarction of rats provides a basis for further study.
