中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2010年
19期
7-8,11
,共3页
bcl-2%cyclinD1%膀胱移行细胞癌%逆转录聚合酶链反应
bcl-2%cyclinD1%膀胱移行細胞癌%逆轉錄聚閤酶鏈反應
bcl-2%cyclinD1%방광이행세포암%역전록취합매련반응
bcl-2%cyclinD1%Bladder transitional cell carcinoma%Reverse transcription polymerase chain reaction
目的 检测bcl-2及cyclinD1 mRNA在膀胱移行细胞癌中的表达.方法 应用逆转录聚合酶链反应(RT-PCR)的方法检测40例膀胱移行细胞癌组织和10例正常膀胱组织中bcl-2、cyclinD1 mRNA的表达情况.结果 40例膀胱移行细胞癌组织中bcl-2、cyclinD1 mRNA的表达分别为0.650±0.018、0.660±0.022,与其在正常膀胱组织中的表达0.250±0.035、0.320±0.028相比,差异均有统计学意义(P＜0.05).Bcl-2 mRNA在临床分期Tα～T2a中的表达(0.720±0.011)显著高于T2b～T4(0.59±0.017),P＜0.05;cyclinD1 mRNA在Tα～T2a的表达量(0.590±0.021)显著低于T2b～T4(0.750±0.021),P＜0.05.相关分析示相关系数为r=-0.34,P=0.031.结论 bcl-2、cyclinD1 mRNA在膀胱移行细胞癌中过度表达与肿瘤的发生、发展密切相关;可用于膀胱移行细胞癌的诊断和评价预后;在膀胱移行细胞癌的发展及评价预后中,bcl-2、cyclinD1 mRNA呈现出负相关趋势.
目的 檢測bcl-2及cyclinD1 mRNA在膀胱移行細胞癌中的錶達.方法 應用逆轉錄聚閤酶鏈反應(RT-PCR)的方法檢測40例膀胱移行細胞癌組織和10例正常膀胱組織中bcl-2、cyclinD1 mRNA的錶達情況.結果 40例膀胱移行細胞癌組織中bcl-2、cyclinD1 mRNA的錶達分彆為0.650±0.018、0.660±0.022,與其在正常膀胱組織中的錶達0.250±0.035、0.320±0.028相比,差異均有統計學意義(P＜0.05).Bcl-2 mRNA在臨床分期Tα～T2a中的錶達(0.720±0.011)顯著高于T2b～T4(0.59±0.017),P＜0.05;cyclinD1 mRNA在Tα～T2a的錶達量(0.590±0.021)顯著低于T2b～T4(0.750±0.021),P＜0.05.相關分析示相關繫數為r=-0.34,P=0.031.結論 bcl-2、cyclinD1 mRNA在膀胱移行細胞癌中過度錶達與腫瘤的髮生、髮展密切相關;可用于膀胱移行細胞癌的診斷和評價預後;在膀胱移行細胞癌的髮展及評價預後中,bcl-2、cyclinD1 mRNA呈現齣負相關趨勢.
목적 검측bcl-2급cyclinD1 mRNA재방광이행세포암중적표체.방법 응용역전록취합매련반응(RT-PCR)적방법검측40례방광이행세포암조직화10례정상방광조직중bcl-2、cyclinD1 mRNA적표체정황.결과 40례방광이행세포암조직중bcl-2、cyclinD1 mRNA적표체분별위0.650±0.018、0.660±0.022,여기재정상방광조직중적표체0.250±0.035、0.320±0.028상비,차이균유통계학의의(P＜0.05).Bcl-2 mRNA재림상분기Tα～T2a중적표체(0.720±0.011)현저고우T2b～T4(0.59±0.017),P＜0.05;cyclinD1 mRNA재Tα～T2a적표체량(0.590±0.021)현저저우T2b～T4(0.750±0.021),P＜0.05.상관분석시상관계수위r=-0.34,P=0.031.결론 bcl-2、cyclinD1 mRNA재방광이행세포암중과도표체여종류적발생、발전밀절상관;가용우방광이행세포암적진단화평개예후;재방광이행세포암적발전급평개예후중,bcl-2、cyclinD1 mRNA정현출부상관추세.
Objective To investigate the expression and clinical significance of bcl-2 and cyclinD1 mRNA in bladder transitional cell carcinoma(TCCB). Methods The expression of bcl-2 and cyclinD1 mRNA were detected by RT-PCR in 40 patients with TCCB and 10 normal bladder tissues. Results The expressional level of bcl-2 and cyclinD1 mRNA in 40 TCCB tissues(0.650±0.018,0.660±0.022) were significantly higher than that level in normal tissues(0.250±0.035,0.320±0.028) (P<0.05). The expression of bcl-2 mRNA in the TCCB tissues of clinical stage Tα-T2a was higher than the expression in stage T2b-T4(P<0.05). But cyclinD1 mRNA in the TCCB tissues of clinical stage T2b-T4 was higher than the expression in stage Tα-T2a(P<0.05),and cyclinD1 mRNA and bcl-2 had correlation(P<0.05). Conclusions RT-PCR assay is reliable and sensitive for detecting bcl-2 and cyclinD1 mRNA in carcinoma specimens from TCCB. The high expression of bcl-2 and cyclinD1 mRNA were correlated to the malignancy.
