肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
3期
148-150
,共3页
基因,ECRG2%RNA干扰%HT1080细胞%细胞运动
基因,ECRG2%RNA榦擾%HT1080細胞%細胞運動
기인,ECRG2%RNA간우%HT1080세포%세포운동
Gene,ECRG2%RNA interference%HT1080 cells%Cell movement
目的 探讨ECRG2基因在人类纤维肉瘤侵袭和转移过程中的作用.方法 应用RNA干扰技术抑制内源性ECRG2基因在人类纤维肉瘤HT1080细胞中的表达,建立ECRG2基因在该细胞中的可诱导表达系统,Western blotting方法观察细胞ECRG2蛋白表达水平的变化,细胞划痕实验和Transwell侵袭实验观察HT1080细胞迁移、侵袭能力的变化.结果 Western blotting检测筛选得到敲除内源性ECRG2基因的最佳Tet-on可诱导表达克隆.细胞划痕实验和Transwell侵袭实验发现,敲除内源性ECRG2基因明显促进HT1080细胞的侵袭迁移能力,而表达外源ECRG2基因的HT1080细胞侵袭、迁移能力显著下降.结论 ECRG2基因与人类纤维肉瘤的侵袭转移潜能相关,外源ECRG2基因的表达可明显抑制人类纤维肉瘤HT1080细胞的侵袭、迁移能力.
目的 探討ECRG2基因在人類纖維肉瘤侵襲和轉移過程中的作用.方法 應用RNA榦擾技術抑製內源性ECRG2基因在人類纖維肉瘤HT1080細胞中的錶達,建立ECRG2基因在該細胞中的可誘導錶達繫統,Western blotting方法觀察細胞ECRG2蛋白錶達水平的變化,細胞劃痕實驗和Transwell侵襲實驗觀察HT1080細胞遷移、侵襲能力的變化.結果 Western blotting檢測篩選得到敲除內源性ECRG2基因的最佳Tet-on可誘導錶達剋隆.細胞劃痕實驗和Transwell侵襲實驗髮現,敲除內源性ECRG2基因明顯促進HT1080細胞的侵襲遷移能力,而錶達外源ECRG2基因的HT1080細胞侵襲、遷移能力顯著下降.結論 ECRG2基因與人類纖維肉瘤的侵襲轉移潛能相關,外源ECRG2基因的錶達可明顯抑製人類纖維肉瘤HT1080細胞的侵襲、遷移能力.
목적 탐토ECRG2기인재인류섬유육류침습화전이과정중적작용.방법 응용RNA간우기술억제내원성ECRG2기인재인류섬유육류HT1080세포중적표체,건립ECRG2기인재해세포중적가유도표체계통,Western blotting방법관찰세포ECRG2단백표체수평적변화,세포화흔실험화Transwell침습실험관찰HT1080세포천이、침습능력적변화.결과 Western blotting검측사선득도고제내원성ECRG2기인적최가Tet-on가유도표체극륭.세포화흔실험화Transwell침습실험발현,고제내원성ECRG2기인명현촉진HT1080세포적침습천이능력,이표체외원ECRG2기인적HT1080세포침습、천이능력현저하강.결론 ECRG2기인여인류섬유육류적침습전이잠능상관,외원ECRG2기인적표체가명현억제인류섬유육류HT1080세포적침습、천이능력.
Objective To investigate the role of ECRG2 gene in migration and invasion of human fibrosarcoma HT1080 cells. Methods Tet-on system was used to set up ECRG2-inducible cell clones.Knockdown of ECRG2 gene was done by stably expressing short hairpin RNA (shRNA) specific for ECRG2 mRNA in a highly invasive fibrosarcoma cell line HT1080. The expressions of ECRG2 proteins were detected by Western blotting assay. Wound-healing scrape assay and transwell invasion assay were performed to assess the effect of ECRG2 gene on the invasive properties of HT1080 in vitro. Results The most effective ECRG2siRNA interference and inducible was selected by Western blotting assay. Knockdown of ECRG2 gene significantly promoted the migration and invasion of HT1080 cells while expression of ECRG2 impaired the migration and invasion of HT1080 cells in vitro. Conclusion ECRG2 gene is correlated with the invasive and metastatic potential of fibrosarcoma carcinoma, and expression of ECRG2 induces the inhibition of migration and invasion of HT1080 cell line.
