CAJ | 학술논문

目的观察低强度电磁场(LIEMF)在促进表皮干细胞(ESCs)移植修复皮肤缺损中的作用.方法取7～8周龄的裸鼠50只,制作全层皮肤缺损后根据随机数字表随机分为3个实验组(1 Hz组、10 Hz组和50 Hz组)和2个对照组(细胞悬液对照组和空白对照组),每组10只.3个实验组和细胞悬液对照组利用胶原海绵将体外分离培养的人包皮来源的ESCs植入裸鼠创面,实验组外加LIEMF(磁感应强度5 mT,频率分别为1 Hz、10 Hz和50 Hz)刺激15 d(每日1次,每次30 min);空白对照组置于同等条件下不加细胞悬液和电磁场.通过计算创面收缩率,光镜下观察组织切片HE染色和免疫组织化学染色结果,以及透射电镜下观察再生皮肤组织内部结构,评定皮肤缺损修复情况.结果 ESCs可以在裸鼠皮肤缺损处成功生长,再生表皮层中有人β1整合素强阳性表达,并且实验组创面收缩率均高于对照组(P＜0.05).光镜和电镜下观察到,LIEMF干预后,各实验组再生皮肤具备完整的表皮层和真皮层,细胞层次增多,表皮层明显增厚,附件丰富,表皮细胞间有桥粒、半桥粒、粗张力微丝.结论 LIEMF可以促进移植了人表皮干细胞的裸鼠全层皮肤缺损创面愈合.
목적 관찰저강도전자장(LIEMF)재촉진표피간세포(ESCs)이식수복피부결손중적작용.방법 취7～8주령적라서50지,제작전층피부결손후근거수궤수자표수궤분위3개실험조(1 Hz조、10 Hz조화50 Hz조)화2개대조조(세포현액대조조화공백대조조),매조10지.3개실험조화세포현액대조조이용효원해면장체외분리배양적인포피래원적ESCs식입라서창면,실험조외가LIEMF(자감응강도5 mT,빈솔분별위1 Hz、10 Hz화50 Hz)자격15 d(매일1차,매차30 min);공백대조조치우동등조건하불가세포현액화전자장.통과계산창면수축솔,광경하관찰조직절편HE염색화면역조직화학염색결과,이급투사전경하관찰재생피부조직내부결구,평정피부결손수복정황.결과 ESCs가이재라서피부결손처성공생장,재생표피층중유인β1정합소강양성표체,병차실험조창면수축솔균고우대조조(P＜0.05).광경화전경하관찰도,LIEMF간예후,각실험조재생피부구비완정적표피층화진피층,세포층차증다,표피층명현증후,부건봉부,표피세포간유교립、반교립、조장력미사.결론 LIEMF가이촉진이식료인표피간세포적라서전층피부결손창면유합.
Objective To observe the effects of low intensity electromagnetic fields (LIEMFs) in promoting the reconstruction of full skin loss wounds grafted with human epidermal stem cells (ESCs). Methods Fifty nude mice aged 7 to 8 weeks with full skin loss wounds were equally divided into 3 experimental subgroups ( 1 Hz, 10Hz and 50Hz) and two control groups (a cell suspension control group and a blank control group) , with ten mice each. In the 3 experimental subgroups and the cell suspension control group, ESCs separated from human foreskin and cultured in vitro were grafted to the wounds using collagen sponge scaffolds. The experimental subgroups were then stimulated with an LIEMF (magnetic field intensity 5mT) at the appropriate frequency for 30min/day for 15 days. The blank control group was put under the same conditions without the cell suspension and LIEMF. The healing rates of the wounds were observed, and tissue slices were stained and observed under a light microscope. The inner structure of the regenerating skin was observed using transmission electron microscopy. Results The ESCs were successfully grafted. A few human integrin β1 positive stained cells appeared in the regenerating skin. The average healing rates in the experimental subgroups were significantly superior to those of the control groups. Well differentiated epidermis and dermis could be seen in the regenerating skin in all of the experimental groups. The epidermis had more cell layers and was thicker than in the control groups. More desmosome, hemidesmosome and keratin filaments were seen among the epidemic cells of the experimental groups. Conclusions LIEMF promotes the healing of full skin loss wounds grafted with ESCs in nude mice, and can promote complete repair of skin defects and the regeneration of skin function.