CAJ | 학술논문

目的探讨人食管鳞状细胞癌中Tollip对Src家族酪氨酸激酶Fyn表达的负调控机制.方法收集3例新鲜食管鳞状细胞癌组织(ESCC)和食管正常黏膜上皮组织(UNR),采用免疫印迹法分析Tollip蛋白表达水平的变化.同时转染人Tollip质粒DNA人TE1细胞株中观察其对Fyn表达的影响.结果　免疫印迹结果　显示食管鳞癌组织Tollip蛋白表达低于食管正常黏膜组织.转染Tollip质粒DNA可以降低人食管鳞状细胞癌TE1细胞中Fyn的表达.结论 Tollip可能是人食管鳞状细胞癌中Src家族酪氨酸激酶ryn的负调控因子,在食管鳞状细胞癌的发生发展过程中起重要作用.
목적 탐토인식관린상세포암중Tollip대Src가족락안산격매Fyn표체적부조공궤제.방법 수집3례신선식관린상세포암조직(ESCC)화식관정상점막상피조직(UNR),채용면역인적법분석Tollip단백표체수평적변화.동시전염인Tollip질립DNA인TE1세포주중관찰기대Fyn표체적영향.결과　면역인적결과　현시식관린암조직Tollip단백표체저우식관정상점막조직.전염Tollip질립DNA가이강저인식관린상세포암TE1세포중Fyn적표체.결론 Tollip가능시인식관린상세포암중Src가족락안산격매ryn적부조공인자,재식관린상세포암적발생발전과정중기중요작용.
Objective To determine the relationship between Src family tyrosine kinases Fyn expression and Toll-interacting protein ( Tollip ) in human esophageal squamous cell carcinoma ( ESCC ).Methods The Tollip expression was detected in 3 cases of ESCC and compared to the unremarkable epithelium by Western blotting. Tollip plasmid DNA ( 1 μg) was transfected into TE1 cells and the Fyn/Tollip expression was examined by Western blotting. Results The Tollip expression level was lower in ESCC group than in UNR group. Tollip decreased Fyn protein expression in TE1 cells. Conclusion Tollip may act as a negative regulator of Fyn in human ESCC, and play an important role in human ESCC progress.