CAJ | 학술논문

目的研究发光二极管(LED)发射的非相干性红光对大鼠成骨细胞增殖及分化的影响.方法通过体外培养SD大鼠成骨细胞,将620 nm波长的LED光源置于细胞上方2 cm处,根据红光照射时间将细胞分为对照组、1 J/cm2组、2 J/cm2组及4 J/cm2组,分别给予红光照射0 s、150 s、300 s和600 s,红光照射每天1次.采用CCK-8法检测红光照射后第2,4天时各组细胞增殖活性;采用碱性磷酸酶(ALP)活性试剂盒检测照射后第9天时细胞ALP比活性;采用逆转录聚合酶链反应(RT-PCR)检测各组细胞Ⅰ型胶原(Coila1)、骨钙素(Bglap)及Runx2的表达情况.结果在红光照射后第4天时,发现1 J/cm2组、2 J/cm2组成骨细胞增殖活性较对照组明显增强(P<0.05);在红光照射后第9天时,4 J/cm2组ALP比活性较对照组明显提高(P<0.05);各红光照射组细胞骨钙素及Runx2表达均较对照组明显增强.结论 LED来源的620 nm非相干性红光照射能显著促进成骨细胞增殖及分化.
목적 연구발광이겁관(LED)발사적비상간성홍광대대서성골세포증식급분화적영향.방법 통과체외배양SD대서성골세포,장620 nm파장적LED광원치우세포상방2 cm처,근거홍광조사시간장세포분위대조조、1 J/cm2조、2 J/cm2조급4 J/cm2조,분별급여홍광조사0 s、150 s、300 s화600 s,홍광조사매천1차.채용CCK-8법검측홍광조사후제2,4천시각조세포증식활성;채용감성린산매(ALP)활성시제합검측조사후제9천시세포ALP비활성;채용역전록취합매련반응(RT-PCR)검측각조세포Ⅰ형효원(Coila1)、골개소(Bglap)급Runx2적표체정황.결과 재홍광조사후제4천시,발현1 J/cm2조、2 J/cm2조성골세포증식활성교대조조명현증강(P<0.05);재홍광조사후제9천시,4 J/cm2조ALP비활성교대조조명현제고(P<0.05);각홍광조사조세포골개소급Runx2표체균교대조조명현증강.결론 LED래원적620 nm비상간성홍광조사능현저촉진성골세포증식급분화.
Objective To investigate the effects of 620nm noncoherent red light emitted from light-emitting diode (LED) on proliferation and osteogenic differentiation of rat osteoblasts.Methods Osteoblasts were isolated from SD rat and cultured in vitro.The LED was placed at 2cm above the cell layer.Cells were divided into four groups and each group was irradiated for 0s, 150s, 300s and 600s at energy dose of 0, 1, 2, and 4J/cm2, respectively.Cells were irradiated once every day.Cellular proliferation activities were evaluated by using cell counting kit-8 ( CCK-8 ) at 2nd and 4th d.The alkaline phosphatase (ALP) ratio activity was evaluated by alkaline phosphatase activity kit at the 9th d, and expressions of osteoblast master genes ( Collα1, Bglap and Runx2) were monitored as indicators of osteoblast differentiation by reverse transcription-polymerase chain reaction (RT-PCR) technique.Results In the group irradiated with 1 and 2 J/cm2 proliferation activities of osteoblasts enhanced significantly compared with the control group at the 4th d ( P < 0.05 ).In the group irradiated with 4 J/cm2 ALP ratio activity elevated significantly compared with the control group at the 9th d ( P <0.05 ).In the group of red light irradiation expressions of Bglap and Runx2 enhanced significantly.Conclusion The 620nm nonconherent red light emitted from LED can promote proliferation of osteoblasts and enhance osteogenic differentiation significantly.