CAJ | 학술논문

目的探讨PIAS3表达对人脑胶质瘤TJ905细胞侵袭力的影响及其可能的机制.方法构建PIAS3过表达载体及合成PIAS3 siRNA,转染TJ905细胞,上调或下调TJ905细胞中PIAS3表达水平,Transwell试验检测TJ905细胞的侵袭力,Western blot验证PIAS3表达及基质金属蛋白酶抑制因子(TIMP)3、基质金属蛋白酶(MMP)-2、MMP-9蛋白的表达.结果体外转染质粒和寡聚核苷酸效率分别为85.3%±3.1%和95.1%±2.9%.体外转染PIAS3过表达质粒能有效提高TJ905细胞中PIAS3蛋白的表达,明显抑制TJ905细胞侵袭力(P<0.05),细胞穿过率由对照组87.9%±9.3%降为37.3%±7.9%,同时上调TIMP3和下调MMP-2、MMP-9蛋白的表达(P<0.05);转染PIAS3 siRNA能有效抑制TJ905细胞中PIAS3蛋白的表达,增强TJ905细胞侵袭力(P<0.05),细胞穿过率由对照组83.9%±7.1%增加到93.2%±3.1%,同时下调TIMP3和上调MMP-2、MMP-9蛋白的表达(P<0.05).结论 PIAS3表达水平与胶质瘤TJ905细胞的侵袭特性密切相关.
목적 탐토PIAS3표체대인뇌효질류TJ905세포침습력적영향급기가능적궤제.방법 구건PIAS3과표체재체급합성PIAS3 siRNA,전염TJ905세포,상조혹하조TJ905세포중PIAS3표체수평,Transwell시험검측TJ905세포적침습력,Western blot험증PIAS3표체급기질금속단백매억제인자(TIMP)3、기질금속단백매(MMP)-2、MMP-9단백적표체.결과 체외전염질립화과취핵감산효솔분별위85.3%±3.1%화95.1%±2.9%.체외전염PIAS3과표체질립능유효제고TJ905세포중PIAS3단백적표체,명현억제TJ905세포침습력(P<0.05),세포천과솔유대조조87.9%±9.3%강위37.3%±7.9%,동시상조TIMP3화하조MMP-2、MMP-9단백적표체(P<0.05);전염PIAS3 siRNA능유효억제TJ905세포중PIAS3단백적표체,증강TJ905세포침습력(P<0.05),세포천과솔유대조조83.9%±7.1%증가도93.2%±3.1%,동시하조TIMP3화상조MMP-2、MMP-9단백적표체(P<0.05).결론 PIAS3표체수평여효질류TJ905세포적침습특성밀절상관.
Objectives To investigate the function and possible mechanisms of PIAS3 expression on the invasion of TJ905 cells. Methods PIAS3 overexpression vectors were constructed and PIAS3 siRNA were chemically synthesized, which were separately transfected into TJ905 cells for upregulation or downregulation of PIAS3 expression levels in TJ905 cells. After that, the invasive effects of TJ905 cells were measured by Transwell assay, and the expression of PIAS3, tissue inhibitor of metalloproteinases (TIMP)3, matrix metalloprotease (MMP)-2, and MMP-9 were identified by Western blot. Results In vitro transfection efficiency of plasmids and oligonucleotides were separately 85.3% ± 3. 1% and 95. 1% ± 2. 9%. PIAS3 overexpression plasmid transfection in vitro could effectively improve the expression of PIAS3 protein in TJ905 cells and inhibit the invasion of TJ905 cells (P < 0. 05), and cell penetration ratio reduced from 87.9% ±9.3% to 37.3% ±7.9% compared with control group, while it upregulated TIMP3 and downregulated MMP-2, MMP-9 protein expression (P<0.05); PIAS3 siRNA transfection could inhibit the PIAS3 protein expression of TJ905 cells and promote the invasion of TJ905 cells (P < 0. 05) , and cell penetration ratio increased from 83. 9% ±7. 1% to 93. 2% ±3. 1% compared with control group, while it downregulated TIMP3 and upregulated MMP-2, MMP-9 protein expression (P < 0.05). Conclusion PIAS3 expression is closely related to the invasion properties of glioma TJ905 cells.