国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2011年
14期
1684-1687
,共4页
放射增敏%温和性高温%G2/M期%cvclinB1%鼻咽癌细胞系CNE-2Z
放射增敏%溫和性高溫%G2/M期%cvclinB1%鼻嚥癌細胞繫CNE-2Z
방사증민%온화성고온%G2/M기%cvclinB1%비인암세포계CNE-2Z
Radiosensitization%Hyperthermia%G2/M phase%CyclinBl%Nasopharyngeal carcinoma cell line CNE-2
目的 探讨温和性高温对鼻咽癌细胞系CNE-2Z放射增敏作用的机制.方法 实验分4个组,对照组、单纯加热组、单纯照射组和联合处理组.采用流式细胞仪分析G2/M期分布比例及凋亡百分比,Western-Blotting检测CyclinB1的表达,改良的荧光染色法观察核碎片形态.结果 联合处理组G2/M期分布比例较高,CvclinB1表达较高,并产生更多的核碎片,放射诱导的凋亡百分比并不增加.结论 G2/M 期分布比例增高,CyclinB1表达增加是温和性高温对鼻咽癌细胞系CNE-2Z放射增敏作用的机制.
目的 探討溫和性高溫對鼻嚥癌細胞繫CNE-2Z放射增敏作用的機製.方法 實驗分4箇組,對照組、單純加熱組、單純照射組和聯閤處理組.採用流式細胞儀分析G2/M期分佈比例及凋亡百分比,Western-Blotting檢測CyclinB1的錶達,改良的熒光染色法觀察覈碎片形態.結果 聯閤處理組G2/M期分佈比例較高,CvclinB1錶達較高,併產生更多的覈碎片,放射誘導的凋亡百分比併不增加.結論 G2/M 期分佈比例增高,CyclinB1錶達增加是溫和性高溫對鼻嚥癌細胞繫CNE-2Z放射增敏作用的機製.
목적 탐토온화성고온대비인암세포계CNE-2Z방사증민작용적궤제.방법 실험분4개조,대조조、단순가열조、단순조사조화연합처리조.채용류식세포의분석G2/M기분포비례급조망백분비,Western-Blotting검측CyclinB1적표체,개량적형광염색법관찰핵쇄편형태.결과 연합처리조G2/M기분포비례교고,CvclinB1표체교고,병산생경다적핵쇄편,방사유도적조망백분비병불증가.결론 G2/M 기분포비례증고,CyclinB1표체증가시온화성고온대비인암세포계CNE-2Z방사증민작용적궤제.
Objective To study the mechanism of radiosensitization of hyperthermia on nasopharyngeal carcinoma cell line CNE-2Z. Methods 4 groups were involved in the experiment: the control group, the simple heat group, the simple radiation group and heat plus radiation group. The change of the distribution of G2/M phase was analyzed by the flow cytometry (FCM), and proportion of apoptosis in addition. Western-Blotting was used to detect the expressions of CyclinBl. Microscope was used to observe the morphology of cells stained with Hoechst 33342. Results More distribution of G2/M phase, along with higher expression of cyclinB1, was found in the heat plus radiation group,leading to more nuclear fragments. Hyperthermia did not add to more apoptosis. Conclusions More distribution of G2/M phase, along with higher expression of cyclinB1 might be the mechanism of radiosensitization of hyperthermia on nasopharyngeal carcinoma cell line CNE-2Z.
