中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2009年
6期
393-395
,共3页
黄珊珊%薛斯亮%杨保华%李利
黃珊珊%薛斯亮%楊保華%李利
황산산%설사량%양보화%리리
皮肤哀老%表皮%基因,p63%抗原,CD44
皮膚哀老%錶皮%基因,p63%抗原,CD44
피부애로%표피%기인,p63%항원,CD44
Skin aging%Epidermis%Genes,p63%Antigens,CD44
目的 探讨p63与CD44在皮肤衰老过程中与年龄、紫外线的关系.方法 采用免疫组化方法检测121例正常全厚皮肤标本中p63与CD44的表达,光镜下观察并计算表皮基底层p63阳性细胞百分比,用图像分析软件image proplus 6.0测量表皮层p63与CD44表达的吸光度值.结果 ①表皮基底层p63阳性细胞百分比与年龄呈负相关(r=-0.218,P＜0.05),51～79岁组最低;但在曝光部位、半曝光部位与非曝光部位间的差异无统计学意义.②表皮p63的表达强度与年龄在曝光部位呈负相关(r=-0.389,P=0.010),在半曝光部位无相关性,在非曝光部位呈正相关(r=0.341,P＜0.05);三个部位表达强度的差异在10～30岁组无统计学意义,在31～50岁组与51～79岁组由强至弱依次为非曝光部位、半曝光部位、曝光部位.③表皮CD44的表达强度与年龄之间呈微弱负相关(r=-0.083,P＜0.05),10～30岁组最高;但在曝光部位、半曝光部位与非曝光部位间的差异无统计学意义.结论 表皮基底层p63阳性细胞百分比、表皮CD44的表达强度和年龄呈负相关,和紫外线照射量无关.表皮p63表达强度在31～50岁组与51～79岁组的表达强度依曝光多少而递减.
目的 探討p63與CD44在皮膚衰老過程中與年齡、紫外線的關繫.方法 採用免疫組化方法檢測121例正常全厚皮膚標本中p63與CD44的錶達,光鏡下觀察併計算錶皮基底層p63暘性細胞百分比,用圖像分析軟件image proplus 6.0測量錶皮層p63與CD44錶達的吸光度值.結果 ①錶皮基底層p63暘性細胞百分比與年齡呈負相關(r=-0.218,P＜0.05),51～79歲組最低;但在曝光部位、半曝光部位與非曝光部位間的差異無統計學意義.②錶皮p63的錶達彊度與年齡在曝光部位呈負相關(r=-0.389,P=0.010),在半曝光部位無相關性,在非曝光部位呈正相關(r=0.341,P＜0.05);三箇部位錶達彊度的差異在10～30歲組無統計學意義,在31～50歲組與51～79歲組由彊至弱依次為非曝光部位、半曝光部位、曝光部位.③錶皮CD44的錶達彊度與年齡之間呈微弱負相關(r=-0.083,P＜0.05),10～30歲組最高;但在曝光部位、半曝光部位與非曝光部位間的差異無統計學意義.結論 錶皮基底層p63暘性細胞百分比、錶皮CD44的錶達彊度和年齡呈負相關,和紫外線照射量無關.錶皮p63錶達彊度在31～50歲組與51～79歲組的錶達彊度依曝光多少而遞減.
목적 탐토p63여CD44재피부쇠로과정중여년령、자외선적관계.방법 채용면역조화방법검측121례정상전후피부표본중p63여CD44적표체,광경하관찰병계산표피기저층p63양성세포백분비,용도상분석연건image proplus 6.0측량표피층p63여CD44표체적흡광도치.결과 ①표피기저층p63양성세포백분비여년령정부상관(r=-0.218,P＜0.05),51～79세조최저;단재폭광부위、반폭광부위여비폭광부위간적차이무통계학의의.②표피p63적표체강도여년령재폭광부위정부상관(r=-0.389,P=0.010),재반폭광부위무상관성,재비폭광부위정정상관(r=0.341,P＜0.05);삼개부위표체강도적차이재10～30세조무통계학의의,재31～50세조여51～79세조유강지약의차위비폭광부위、반폭광부위、폭광부위.③표피CD44적표체강도여년령지간정미약부상관(r=-0.083,P＜0.05),10～30세조최고;단재폭광부위、반폭광부위여비폭광부위간적차이무통계학의의.결론 표피기저층p63양성세포백분비、표피CD44적표체강도화년령정부상관,화자외선조사량무관.표피p63표체강도재31～50세조여51～79세조적표체강도의폭광다소이체감.
Objective To clarify the relationship of p63 and CD44 expressions with age and ultraviolet irradiation in the process of skin aging.Methods Full-thickness skin samples were obtained from the normal skin at different sites,including 44 sun-exposed sites(face,neck,dorsal aspect of the forearm),46 sun-protected sites(abdomen,thigh),and 31 semi-exposed sites(back,flexor aspect of the forearm,upper arm,leg),of 121 volunteers(63 males and 58 females).Of all the volunteers,55 were 10 t0 30 vears of age (young),41 were 31 to 50 years of age(adult),and 25 were 51 to 79 years of age(old).Immunohistochemisty using antibodies to p63 and CD44 was applied to detect the expression of p63 and CD44 in these specimens.Imaging analysis software was utilized to calculate the percentage of p63-expressing cells in basal layer as well as the expression level of p63 and CD44.Results In stratum basale,the percentage of p63-expressing cells decreased with age(r=-0.218,P＜0.05),but showed no significant difference among sun-exposed skin,semi-exposed skin and sun-protected skin(P＞0.05).The correlation between the expression intensity of epidermal p63 and age was negative in sun-exposed sites(r=-0.389,P=0.010),positive in sun-protected sites(r=0.341,P＜0.05).but non-significant in semi-exposed sites(P＞0.05).With regard to the expression of epidermal p63,no significant difference was observed among sun-exposed sites,sun-protected sites and semi-exposed sites in volunteers aged from 10 to 30 years.while in those aged from 31 to 50 years and from 51 to 79 years.sun-protected sites was significantly higher than semi-exposed sites followed by sun-exposed sites(all P＜0.05).There was a weak correlation between the expression intensity of epidermal CD44 and age(r=-0.083,P＜0.05),but no significant difierence was noted among the three kinds of sites(P＞0.05).Conclusions In stratum basale,the percentage of p63.expressing cells and the expression intensity Of CD44 negatively correlate with age,but have no correlation with ultraviolet irradiation.In individuals aged from 31 to 50 years and from 51 t0 79 vears.the expression of epidermal p63 decreases with the accumulation of sun-exposure.
