中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2010年
2期
123-127
,共5页
桑道乾%李志军%陈琳洁%段京明
桑道乾%李誌軍%陳琳潔%段京明
상도건%리지군%진림길%단경명
关节炎,类风湿%多态性,单核苷酸%单倍性
關節炎,類風濕%多態性,單覈苷痠%單倍性
관절염,류풍습%다태성,단핵감산%단배성
Arthritis,rheumatoid%Polymorphism,single nucleotide%Haploidy
目的 探讨Fc受体样基因3(FeRL3)部分单核苷酸多态性(SNP)在安徽籍汉族类风湿关节炎(RA)发病中的作用.方法 选取RA患者140例,健康对照组187名.用Mass-ArrayTM SNP检测仪对FcRL3基因上的SNP位点:FeRL3-1(rs0158440)、FcRL3-2(rs2225828)、FcRL3-3(rs7528684)、FcRL3-4(rs11284799)、FeRL3-5(rs945635)、FcRL3-6(rs3761959)、FcRL3-7(rs2210913)、FcRL3-8(rs2282284)、FcRL3-9(rs2282283)SNP进行检测.单倍型构建采用PHASE v 2.1软件完成.结果 在RA组中FcRL3-3(rs7528684)-169C等位基因的频率为43.80%,显著高于对照组33.33%.差异有统计学意义(X~2=7.348,P=0.007,OR=1.558,CI:1.130~2.150).单倍型FcRL3-1~9 CCCGGGGAA、CTCGCAAAC、CCTACAAAA、CCTGCGGAA、CTCGCGAAC差异有统计学意义,其中RA组中FcRL3-1~9 CCCGCAGGAA、CTCGCAAAC单倍型频率明显高于对照组,FcRL3-1~9 CCTACAAAA、CCTGCGGAA、CTCGCGAAC单倍型频率明显低于对照组.结论 FcRL3基因可能与安徽籍汉族RA的易感性相关.
目的 探討Fc受體樣基因3(FeRL3)部分單覈苷痠多態性(SNP)在安徽籍漢族類風濕關節炎(RA)髮病中的作用.方法 選取RA患者140例,健康對照組187名.用Mass-ArrayTM SNP檢測儀對FcRL3基因上的SNP位點:FeRL3-1(rs0158440)、FcRL3-2(rs2225828)、FcRL3-3(rs7528684)、FcRL3-4(rs11284799)、FeRL3-5(rs945635)、FcRL3-6(rs3761959)、FcRL3-7(rs2210913)、FcRL3-8(rs2282284)、FcRL3-9(rs2282283)SNP進行檢測.單倍型構建採用PHASE v 2.1軟件完成.結果 在RA組中FcRL3-3(rs7528684)-169C等位基因的頻率為43.80%,顯著高于對照組33.33%.差異有統計學意義(X~2=7.348,P=0.007,OR=1.558,CI:1.130~2.150).單倍型FcRL3-1~9 CCCGGGGAA、CTCGCAAAC、CCTACAAAA、CCTGCGGAA、CTCGCGAAC差異有統計學意義,其中RA組中FcRL3-1~9 CCCGCAGGAA、CTCGCAAAC單倍型頻率明顯高于對照組,FcRL3-1~9 CCTACAAAA、CCTGCGGAA、CTCGCGAAC單倍型頻率明顯低于對照組.結論 FcRL3基因可能與安徽籍漢族RA的易感性相關.
목적 탐토Fc수체양기인3(FeRL3)부분단핵감산다태성(SNP)재안휘적한족류풍습관절염(RA)발병중적작용.방법 선취RA환자140례,건강대조조187명.용Mass-ArrayTM SNP검측의대FcRL3기인상적SNP위점:FeRL3-1(rs0158440)、FcRL3-2(rs2225828)、FcRL3-3(rs7528684)、FcRL3-4(rs11284799)、FeRL3-5(rs945635)、FcRL3-6(rs3761959)、FcRL3-7(rs2210913)、FcRL3-8(rs2282284)、FcRL3-9(rs2282283)SNP진행검측.단배형구건채용PHASE v 2.1연건완성.결과 재RA조중FcRL3-3(rs7528684)-169C등위기인적빈솔위43.80%,현저고우대조조33.33%.차이유통계학의의(X~2=7.348,P=0.007,OR=1.558,CI:1.130~2.150).단배형FcRL3-1~9 CCCGGGGAA、CTCGCAAAC、CCTACAAAA、CCTGCGGAA、CTCGCGAAC차이유통계학의의,기중RA조중FcRL3-1~9 CCCGCAGGAA、CTCGCAAAC단배형빈솔명현고우대조조,FcRL3-1~9 CCTACAAAA、CCTGCGGAA、CTCGCGAAC단배형빈솔명현저우대조조.결론 FcRL3기인가능여안휘적한족RA적역감성상관.
Objective To investigate the association between a few single nucleotide polymorphisms (SNPs)in Fc receptor like 3(FcRL3)and rheumatoid arthritis(RA)in Han nationality of Anhui patients.Methods One hundred and forty RA patients along with one hundred and eighty-seven healthy controls were included in the study.SNPs of FcRL3-1(rs0158440),FcRL3-2(rs2225828),FcRL3-3(rs7528684).FcRL34(rs11284799),FcRL3-5(rs945635),FcRL3-6(rs3761959),FcRL3-7(rs2210913),FcRL3-8(rs2282284)and FcRL3-9(rs2282283)in the FcRL3 gene were genotyped by MALDI-TOF technology.Haplotypes were estimated using PHASE v 2.1 software.Results The frequency of FcRL3-3-1 69C alleles in RA patients was significantly increased compared with healthy controls(X~2=7.348,P=0.007,OR=1.558,CI:1.130~2.150).The decreased compared with healthy controls.ConclusionFcRL3 may be associated with RA susceptibility in Anhui Han population.