中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
5期
261-266
,共6页
郭红英%朱慧%张正国%屈莉红%肖宏%冯艳玲%向明%李方明%胡祥%王介非
郭紅英%硃慧%張正國%屈莉紅%肖宏%馮豔玲%嚮明%李方明%鬍祥%王介非
곽홍영%주혜%장정국%굴리홍%초굉%풍염령%향명%리방명%호상%왕개비
大鼠,Wistar%肝功能衰竭,急性%人类%脐带%间质干细胞移植
大鼠,Wistar%肝功能衰竭,急性%人類%臍帶%間質榦細胞移植
대서,Wistar%간공능쇠갈,급성%인류%제대%간질간세포이식
Rats,Wistar%Liver failure,acute%Human%Umbilical cords%Mesenchymal stem cell transplantation
目的 探讨人脐带间充质干细胞(hUCMSCs)对急性肝功能衰竭大鼠的治疗效果.方法 腹腔注射D-氨基半乳糖(D-gal)和脂多糖(LPS)诱导大鼠急性肝功能衰竭.将造模成功的大鼠经腹腔或尾静脉移植hUCMSCs,同时将注射0.9%氯化钠溶液的大鼠作为对照组.在第0、1、2、3、5、7天分别检测ALT、TBil和Alb,第7天处死大鼠,观察其肝脏组织病理学改变.统计学方法采用Fisher确切概率法、非配对t检验和Mann-Whitney U检验.结果 腹腔或尾静脉移植hUCMSCs组大鼠存活率与对照组相比,差异均无统计学意义(Fisher确切概率法,均P=1.00).腹腔移植hUCMSCs组大鼠移植后2 d ALT、TBil、Alb分别为(804.9±88.0)U/L、(17.4±2.7)μmol/L、(20.9±0.8)g/L,对照组分别为(1294.3±171.4)U/L、(32.3±5.5)μmol/L、(16.1±0.9)g/L(t=2.640,P=0.020;r=2.529,P=0.025;t=-3.833,P=0.002).尾静脉移植hUCMNCs组肝功能指标改善情况与腹腔移植组相似.腹腔和尾静脉移植hUCMSCs组肝组织损伤程度比对照组轻(U=4.500,P=0.005;U=4.500,P=0.008),肝细胞分裂相指数也有显著提高(U=4.000,P=0.005;U=5.500,P=0.013).结论 经腹腔或尾静脉移植hUCMSCs均可促进急性肝功能衰竭大鼠ALT、TBil、Alb等指标恢复正常,新生肝细胞增殖活跃,同时明显改善肝组织损伤病理分级.
目的 探討人臍帶間充質榦細胞(hUCMSCs)對急性肝功能衰竭大鼠的治療效果.方法 腹腔註射D-氨基半乳糖(D-gal)和脂多糖(LPS)誘導大鼠急性肝功能衰竭.將造模成功的大鼠經腹腔或尾靜脈移植hUCMSCs,同時將註射0.9%氯化鈉溶液的大鼠作為對照組.在第0、1、2、3、5、7天分彆檢測ALT、TBil和Alb,第7天處死大鼠,觀察其肝髒組織病理學改變.統計學方法採用Fisher確切概率法、非配對t檢驗和Mann-Whitney U檢驗.結果 腹腔或尾靜脈移植hUCMSCs組大鼠存活率與對照組相比,差異均無統計學意義(Fisher確切概率法,均P=1.00).腹腔移植hUCMSCs組大鼠移植後2 d ALT、TBil、Alb分彆為(804.9±88.0)U/L、(17.4±2.7)μmol/L、(20.9±0.8)g/L,對照組分彆為(1294.3±171.4)U/L、(32.3±5.5)μmol/L、(16.1±0.9)g/L(t=2.640,P=0.020;r=2.529,P=0.025;t=-3.833,P=0.002).尾靜脈移植hUCMNCs組肝功能指標改善情況與腹腔移植組相似.腹腔和尾靜脈移植hUCMSCs組肝組織損傷程度比對照組輕(U=4.500,P=0.005;U=4.500,P=0.008),肝細胞分裂相指數也有顯著提高(U=4.000,P=0.005;U=5.500,P=0.013).結論 經腹腔或尾靜脈移植hUCMSCs均可促進急性肝功能衰竭大鼠ALT、TBil、Alb等指標恢複正常,新生肝細胞增殖活躍,同時明顯改善肝組織損傷病理分級.
목적 탐토인제대간충질간세포(hUCMSCs)대급성간공능쇠갈대서적치료효과.방법 복강주사D-안기반유당(D-gal)화지다당(LPS)유도대서급성간공능쇠갈.장조모성공적대서경복강혹미정맥이식hUCMSCs,동시장주사0.9%록화납용액적대서작위대조조.재제0、1、2、3、5、7천분별검측ALT、TBil화Alb,제7천처사대서,관찰기간장조직병이학개변.통계학방법채용Fisher학절개솔법、비배대t검험화Mann-Whitney U검험.결과 복강혹미정맥이식hUCMSCs조대서존활솔여대조조상비,차이균무통계학의의(Fisher학절개솔법,균P=1.00).복강이식hUCMSCs조대서이식후2 d ALT、TBil、Alb분별위(804.9±88.0)U/L、(17.4±2.7)μmol/L、(20.9±0.8)g/L,대조조분별위(1294.3±171.4)U/L、(32.3±5.5)μmol/L、(16.1±0.9)g/L(t=2.640,P=0.020;r=2.529,P=0.025;t=-3.833,P=0.002).미정맥이식hUCMNCs조간공능지표개선정황여복강이식조상사.복강화미정맥이식hUCMSCs조간조직손상정도비대조조경(U=4.500,P=0.005;U=4.500,P=0.008),간세포분렬상지수야유현저제고(U=4.000,P=0.005;U=5.500,P=0.013).결론 경복강혹미정맥이식hUCMSCs균가촉진급성간공능쇠갈대서ALT、TBil、Alb등지표회복정상,신생간세포증식활약,동시명현개선간조직손상병리분급.
Objective To study the therapeutic effects of human umbilical cord mesenchymal stem cells (hUCMSCs) on acute liver failure ( ALF) induced by D-galactosamine (D-gal) and lipopolysaccharide (LPS) in rats. Methods The ALF model was obtained through intraperitoneal injection of D-gal(300 mg/kg)and LPS (20μg/kg)in Wister rats. The hUCMSCs were transplanted after intoxication. All rats were divided into four groups, and each group received either hUCMSCs or 0.9% NaCl solution through intraperitoneal or tail-intravenous injection. To evaluate the liver function of each group, the levels of alanine aminotransferase (ALT), total bilirubin (TBil) and serum albumin (Alb) were measured on the day of hUCMSCs transplantation and the following 1, 2, 3, 5 and 7 days. All rats were then sacrificed to examine the liver histology at day 7. Analyses were done by using Fisher's exact test, unpaired t test and Mann-Whitney U test. Results There were no significant differences of survival rates among four groups (Fisher's exact test, both P = 1. 00). The levels of ALT, TBil and Alb in group receiving hUCMSCs intraperitoneally were (804. 9 ± 88. 0) U/L,(17. 4±2. 7) μmol/L and (20. 9±0. 8) g/L, respectively after 2 days of injection, whereas in the corresponding control group, those were (1294. 3± 171. 4) U/L, (32. 3±5. 5) μmol/L and (16. 1±0. 9) g/L, respectively, which indicated that hUCMSCs transplantation significantly improved the liver function (t = 2. 640, P =0.020;t=2.529, P = 0. 025;t= - 3. 833, P = 0. 002). Both of hUCMSCs-transplanted groups showed no significant differences. Liver histological data showed that transplantation of hUSMSCs through either intraperitoneal or tail-intravenous injection alleviated liver damage (U=4. 500, P = 0. 005;U=4. 500, P = 0. 008) and the mitotic index also increased in hUCMSCs-transplanted groups (U=4. 000, P = 0. 005; U=5. 500, P = 0. 013). Conclusions The levels of ALT, TBil and Alb can rapidly normalize in ALF rats after injected with hUCMSCs either intraperitoneally or tail-intravenously. hUCMSCs application raises the mitotic index, enhances hepatocellular regeneration and improves histological status.