中国临床实用医学
中國臨床實用醫學
중국림상실용의학
CHINA CLINICAL PRACTICAL MEDICINE
2010年
11期
40-43
,共4页
胰岛素样生长因子-Ⅰ%胰岛素样生长因子-Ⅰ受体%糖尿病足溃疡
胰島素樣生長因子-Ⅰ%胰島素樣生長因子-Ⅰ受體%糖尿病足潰瘍
이도소양생장인자-Ⅰ%이도소양생장인자-Ⅰ수체%당뇨병족궤양
Insulin like growth factor Ⅰ%Insulin like growth factor Ⅰ%Diabetic foot ulcers
目的 探讨胰岛素样生长因子-Ⅰ(Insulin-like growth factor-Ⅰ,IGF-Ⅰ)与其受体两亚基(α,β)在非糖尿病正常皮肤和单纯糖尿病患者皮肤,以及糖尿病足患者足溃疡创面中的分布和表达特征,旨在阐明其表达水平变化与糖尿病足溃疡创面的内在联系.方法 按照病例入选标准收集30例糖尿病足患者,23例单纯糖尿病患者,以及25例非糖尿病正常人,取足部皮肤组织标本,经固定脱水,石蜡包埋,切片后备用.采用免疫组织化学SP法检测所有取材组织中IGF-Ⅰ、IGF-Ⅰ Rα和IGF-Ⅰ Rβ蛋白定位及含量.在400倍光镜下观察免疫组化结果.结果 在非糖尿病正常皮肤和单纯糖尿病患者皮肤,以及糖尿病足溃疡创面皮肤中IGF-Ⅰ、IGF-Ⅰ Rα和IGF-Ⅰ Rβ都有阳性表达,但变化规律不尽相同.IGF-Ⅰ的阳性细胞率单纯糖尿病组较正常对照组表达减弱(P＞0.05),糖尿病足溃疡组较单纯糖尿病组表达进一步减弱(P＞0.05).IGF-Ⅰ Rα和IGF-Ⅰ Rβ的阳性细胞率单纯糖尿病组较对照组表达减弱(P＞0.05),糖尿病足溃疡组较单纯糖尿病组表达进一步减弱(P＞0.05).结论 糖尿病患者创面IGF-Ⅰ的缺乏或不足可能与糖尿病所引发的内皮细胞损害有关,与糖尿病足部溃疡经久不愈有直接关系.创面IGF-Ⅰ Rα和IGF-Ⅰ Rβ表达减少,和生物活性的降低,影响细胞因子引起的信号传递和核内目的基因表达,导致创面修复失控形成慢性难愈性溃疡.
目的 探討胰島素樣生長因子-Ⅰ(Insulin-like growth factor-Ⅰ,IGF-Ⅰ)與其受體兩亞基(α,β)在非糖尿病正常皮膚和單純糖尿病患者皮膚,以及糖尿病足患者足潰瘍創麵中的分佈和錶達特徵,旨在闡明其錶達水平變化與糖尿病足潰瘍創麵的內在聯繫.方法 按照病例入選標準收集30例糖尿病足患者,23例單純糖尿病患者,以及25例非糖尿病正常人,取足部皮膚組織標本,經固定脫水,石蠟包埋,切片後備用.採用免疫組織化學SP法檢測所有取材組織中IGF-Ⅰ、IGF-Ⅰ Rα和IGF-Ⅰ Rβ蛋白定位及含量.在400倍光鏡下觀察免疫組化結果.結果 在非糖尿病正常皮膚和單純糖尿病患者皮膚,以及糖尿病足潰瘍創麵皮膚中IGF-Ⅰ、IGF-Ⅰ Rα和IGF-Ⅰ Rβ都有暘性錶達,但變化規律不儘相同.IGF-Ⅰ的暘性細胞率單純糖尿病組較正常對照組錶達減弱(P＞0.05),糖尿病足潰瘍組較單純糖尿病組錶達進一步減弱(P＞0.05).IGF-Ⅰ Rα和IGF-Ⅰ Rβ的暘性細胞率單純糖尿病組較對照組錶達減弱(P＞0.05),糖尿病足潰瘍組較單純糖尿病組錶達進一步減弱(P＞0.05).結論 糖尿病患者創麵IGF-Ⅰ的缺乏或不足可能與糖尿病所引髮的內皮細胞損害有關,與糖尿病足部潰瘍經久不愈有直接關繫.創麵IGF-Ⅰ Rα和IGF-Ⅰ Rβ錶達減少,和生物活性的降低,影響細胞因子引起的信號傳遞和覈內目的基因錶達,導緻創麵脩複失控形成慢性難愈性潰瘍.
목적 탐토이도소양생장인자-Ⅰ(Insulin-like growth factor-Ⅰ,IGF-Ⅰ)여기수체량아기(α,β)재비당뇨병정상피부화단순당뇨병환자피부,이급당뇨병족환자족궤양창면중적분포화표체특정,지재천명기표체수평변화여당뇨병족궤양창면적내재련계.방법 안조병례입선표준수집30례당뇨병족환자,23례단순당뇨병환자,이급25례비당뇨병정상인,취족부피부조직표본,경고정탈수,석사포매,절편후비용.채용면역조직화학SP법검측소유취재조직중IGF-Ⅰ、IGF-Ⅰ Rα화IGF-Ⅰ Rβ단백정위급함량.재400배광경하관찰면역조화결과.결과 재비당뇨병정상피부화단순당뇨병환자피부,이급당뇨병족궤양창면피부중IGF-Ⅰ、IGF-Ⅰ Rα화IGF-Ⅰ Rβ도유양성표체,단변화규률불진상동.IGF-Ⅰ적양성세포솔단순당뇨병조교정상대조조표체감약(P＞0.05),당뇨병족궤양조교단순당뇨병조표체진일보감약(P＞0.05).IGF-Ⅰ Rα화IGF-Ⅰ Rβ적양성세포솔단순당뇨병조교대조조표체감약(P＞0.05),당뇨병족궤양조교단순당뇨병조표체진일보감약(P＞0.05).결론 당뇨병환자창면IGF-Ⅰ적결핍혹불족가능여당뇨병소인발적내피세포손해유관,여당뇨병족부궤양경구불유유직접관계.창면IGF-Ⅰ Rα화IGF-Ⅰ Rβ표체감소,화생물활성적강저,영향세포인자인기적신호전체화핵내목적기인표체,도치창면수복실공형성만성난유성궤양.
Objective This study investigated the distribution and expression of the IGF- Ⅰ and its receptor 2 subunits ( αt, β) in non-diabetic and diabetic patients' normal skin as well as diabetic foot-ulcer, to clarify the expression of level change and diabetic foot ulcer inherent connection. Methods the serum and foot skin of diabetes foot patients (30 cases), diabetes patients (23 cases)and control (25 cases)ware collected. After fixation、dehydration、 embedding、 slicing, the line IGF-1、 IGF-Ⅰ Rαand IGF-Ⅰ Rβimmunohistochemistry. observed the results of immunohischemistry under light microscope at 400 times. Results IGF- Ⅰ、IGF- Ⅰ Rαand IGF- Ⅰ Rβis positive in non-diabetic or diabetic patients' normal skin and diabetic foot ulcer, but the variation is different. IGF- Ⅰ positive cell rate of DM group than in normal control group decreased ( P ＞ 0. 05 ) , Diabetic foot ulcer group than in the expression of further weakening in simple diabetic group (P ＞ 0. 05 ). IGF- Ⅰ Rαand IGF- Ⅰ Rβpositive cell rate of DM group than in normal control group decreased (P ＞ 0. 05 ), Diabetic foot ulcer group than in the expression of further weakening in simple diabetic group ( P ＞ 0. 05 ). Conclusion IGF- Ⅰ diabetic wound the lack or insufficiency may be caused by diabetes, endothelial cell damage, and prolonged healing of diabetic foot ulcers are directly related. Wound expression of IGF- Ⅰ Rαand IGF- Ⅰ Rβ decreased, and biological activity of the reduced impact of cytokine induced signaling and gene expression in the nucleus, resulting in lost control and wound healing of chronic dermal ulcer formation.
