CAJ | 학술논문

目的采用地塞米松肌注法建立糖皮质激素性骨质疏松( glucocorticoid-induced osteoporosis,GIO)大鼠模型,观察补肾中药的防治作用.方法 Wistar大鼠60只,雌雄各半,按体重分层随机法分为正常组、模型组、补肾组.均采用肌注地塞米松的方法造模,2.5 mg/kg·次-1,2次/周,连续9周.测定离体股骨骨密度(BMD)及血清骨代谢指标.结果模型组模型大鼠BMD明显降低(0.109±0.007) g/cm2,血清骨吸收标记物TRAP含量明显升高(9.96±1.15)μg/ml;补肾组模型大鼠BMD(0.116±0.007) g/cm2,血清TRAP含量降低(5.76±0.85) μg/ml.结论补肾中药对糖皮质激素诱导的GIO大鼠模型有一定拮抗作用.
목적 채용지새미송기주법건립당피질격소성골질소송( glucocorticoid-induced osteoporosis,GIO)대서모형,관찰보신중약적방치작용.방법 Wistar대서60지,자웅각반,안체중분층수궤법분위정상조、모형조、보신조.균채용기주지새미송적방법조모,2.5 mg/kg·차-1,2차/주,련속9주.측정리체고골골밀도(BMD)급혈청골대사지표.결과 모형조모형대서BMD명현강저(0.109±0.007) g/cm2,혈청골흡수표기물TRAP함량명현승고(9.96±1.15)μg/ml;보신조모형대서BMD(0.116±0.007) g/cm2,혈청TRAP함량강저(5.76±0.85) μg/ml.결론 보신중약대당피질격소유도적GIO대서모형유일정길항작용.
Objective To observe the effects of nourishing-kidney herbs on rat model with glucocorticoid-induced osteoporosis.Methods 60 Wistar rats male and female half and half were randomly divided into a normal group,a model group and a nourishing-kidney group.By intramuscular injection of dexamethasone (2.5 mg/kg) twice a week to replicate osteoporosis rat model.All groups were treated for 9 weeks.Detected the BMD of femur in vitro and determined the bone metabolism marker in serum by biochemical process.Results The BMD decreased obviously (0.109± 0.007)g/cm2 and the content of TRAP in serum increased evidently (9.96± 1.15) μg/ml in the model group.In the nourishing-kidney group,the BMD was up-regulated (0.116 ± 0.007)g/cm2,and TRAP down-regulated(5.76 ± 0.85)μg/ml.Conclusion Intramuscular injection of dexamethasone can induce GIO rat model,and nourishing-kidney herbs have the effect of anti-osteoporosis.