国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2011年
5期
532-536
,共5页
朱玲玲%张良成%许清秀%赖忠盟%郭永正
硃玲玲%張良成%許清秀%賴忠盟%郭永正
주령령%장량성%허청수%뢰충맹%곽영정
七氟烷%海马%认知功能%脑源性神经营养因子
七氟烷%海馬%認知功能%腦源性神經營養因子
칠불완%해마%인지공능%뇌원성신경영양인자
Sevoflurane%Hippocampus%Cognition function%Brain derived neurotrophic factor
目的 评价七氟烷不同吸入浓度与时间对新生大鼠认知功能和海马脑源性神经营养因子(brain derived neurotrophic factor,BDNF)表达的远期影响.方法 7d龄SD大鼠采用随机数字表法随机分为4组(每组20只):空白对照组(C组)、5.1％七氟烷1.3 h组(S组)、3.4％七氟烷2h组(M组)和1.7％七氟烷4h组(L组).麻醉后21 d进行旷场实验和新异物体识别实验,随后采取免疫组织化学和逆转录-聚合酶链反应(reverse transcriptive polymerase chain reaction,RT-PCR)技术,分别检测各组大鼠海马BDNF和BDNF mRNA的表达.结果 ①各项检测均发现S组与对照组差异无统计学意义(P＞0.05)；②旷场实验发现M组与对照组差异无统计学意义；新异物体识别实验中M组在第24小时的探究新物体时间所占比值为63.4％,明显低于对照组69.8％(P＜0.05)；海马区BDNF平均光密度值和BDNF mRNA的相对表达量分别为0.229±0.021与0.775±0.024,分别低于对照组的0.251 ±0.020与0.799±0.023(P＜0.05).③行为学测试与BDNF和BDNF mRNA检测显示,L组大鼠的各项指标与对照组差异有统计学意义(P＜0.05),同M组相比差异也具有统计学意义(P＜0.05).结论 七氟烷可能导致新生大鼠远期的认知功能下降,其中时间因素影响较大,可能与海马部位BDNF表达下降有关.
目的 評價七氟烷不同吸入濃度與時間對新生大鼠認知功能和海馬腦源性神經營養因子(brain derived neurotrophic factor,BDNF)錶達的遠期影響.方法 7d齡SD大鼠採用隨機數字錶法隨機分為4組(每組20隻):空白對照組(C組)、5.1％七氟烷1.3 h組(S組)、3.4％七氟烷2h組(M組)和1.7％七氟烷4h組(L組).痳醉後21 d進行曠場實驗和新異物體識彆實驗,隨後採取免疫組織化學和逆轉錄-聚閤酶鏈反應(reverse transcriptive polymerase chain reaction,RT-PCR)技術,分彆檢測各組大鼠海馬BDNF和BDNF mRNA的錶達.結果 ①各項檢測均髮現S組與對照組差異無統計學意義(P＞0.05)；②曠場實驗髮現M組與對照組差異無統計學意義；新異物體識彆實驗中M組在第24小時的探究新物體時間所佔比值為63.4％,明顯低于對照組69.8％(P＜0.05)；海馬區BDNF平均光密度值和BDNF mRNA的相對錶達量分彆為0.229±0.021與0.775±0.024,分彆低于對照組的0.251 ±0.020與0.799±0.023(P＜0.05).③行為學測試與BDNF和BDNF mRNA檢測顯示,L組大鼠的各項指標與對照組差異有統計學意義(P＜0.05),同M組相比差異也具有統計學意義(P＜0.05).結論 七氟烷可能導緻新生大鼠遠期的認知功能下降,其中時間因素影響較大,可能與海馬部位BDNF錶達下降有關.
목적 평개칠불완불동흡입농도여시간대신생대서인지공능화해마뇌원성신경영양인자(brain derived neurotrophic factor,BDNF)표체적원기영향.방법 7d령SD대서채용수궤수자표법수궤분위4조(매조20지):공백대조조(C조)、5.1％칠불완1.3 h조(S조)、3.4％칠불완2h조(M조)화1.7％칠불완4h조(L조).마취후21 d진행광장실험화신이물체식별실험,수후채취면역조직화학화역전록-취합매련반응(reverse transcriptive polymerase chain reaction,RT-PCR)기술,분별검측각조대서해마BDNF화BDNF mRNA적표체.결과 ①각항검측균발현S조여대조조차이무통계학의의(P＞0.05)；②광장실험발현M조여대조조차이무통계학의의；신이물체식별실험중M조재제24소시적탐구신물체시간소점비치위63.4％,명현저우대조조69.8％(P＜0.05)；해마구BDNF평균광밀도치화BDNF mRNA적상대표체량분별위0.229±0.021여0.775±0.024,분별저우대조조적0.251 ±0.020여0.799±0.023(P＜0.05).③행위학측시여BDNF화BDNF mRNA검측현시,L조대서적각항지표여대조조차이유통계학의의(P＜0.05),동M조상비차이야구유통계학의의(P＜0.05).결론 칠불완가능도치신생대서원기적인지공능하강,기중시간인소영향교대,가능여해마부위BDNF표체하강유관.
Objective To investigate the long-term effects of sevoflurane given by different concentration and time course on cognitive function and expression of hippocampus brain derived neurotrophic factor(BDNF)in neonatal rats.Methods SD rats at seven-day-old were randomly divided into 4 groups.Rats only inhaled oxygen as control(group C)or inhaled sevoflurane at 5.1％ for 1.3 h(group S),at 3.4％ for 2 h(group M)and at 1.7％ for 4 h(group L).Each group has 20 rats.Open field test and novel obiect recognition test were used to assess the cognitive function of the rats at 28-day-old.Then these rats were killed and measurede the expressions of BDNF and BDNF mRNA using immuno-histochemistry and reverse transcriptive polymerase chain reaction(RT-PCR)in hippocampus respectively.Results ① In all tests,there was no significant change between group S and group C(P＞0.05).② Group M didn't not exhibit significant difference in comparison with group C in every item of the open field test.In the novel obiect recognition test,the percentage of exploring time on new object of group M in 24th hour was 63.4％,which showed significantly lower than controls 69.8％(P＜0.05).The average optic density of BDNF(0.229±0.021)and the relative value of BDNF mRNA(0.775±0.024)of hippocampus of group M were both lower than(0.0251 ±0.020)and(0.799±0.023)of control C respectivly(P＜0.05).③ No matter the tests of cognitive function or expressions of BDNF and BDNF mRNA,each index of group L was worse than group C and group M (P＜0.05).Conclusion sevoflurane can induce forward cognitive decline in neonate rat,that may be because of decreasing the expression of BDNF in hippocampus.The time of inhaleing sevoflurane was an important reason.
