中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2001年
2期
88-90
,共3页
董满库%陈昌玮%崔彦%王平%扬飞%肖广生%任冬云%林京%刘子沛%李晓鸥
董滿庫%陳昌瑋%崔彥%王平%颺飛%肖廣生%任鼕雲%林京%劉子沛%李曉鷗
동만고%진창위%최언%왕평%양비%초엄생%임동운%림경%류자패%리효구
肝缺血%再灌注损伤%内皮素-1
肝缺血%再灌註損傷%內皮素-1
간결혈%재관주손상%내피소-1
目的探索内皮素-1（ET-1）在肝脏缺血再灌注损伤中的作用。方法选用雄性Wistar大鼠80只，分为正常对照组、缺血再灌注组、生理盐水组和ET-1抗体组，观察肝脏缺血60min再灌注3h后血浆ET-1、丙氨酸转氨酶（ALT）、透明质酸（HA）、以及肝组织中ET-1和丙二醛（MDA）含量的变化，并观察肝组织病理学变化，同时，在缺血再灌注组选择第1、3、6、12和24h时相点观察ET-1的变化规律。结果肝脏缺血再灌注后，血浆和肝组织中ET-1，血浆HA、ALT和肝组织中MDA显著升高，而ET-1抗体组血浆ET-1、HA、ALT与缺血再灌注组相比显著降低（P＜0.01,P＜0.05），同时，肝组织的瘀血程度和损伤程度显著改善。结论 ET-1参与了肝脏缺血再灌注损伤，这种损伤与肝脏微循环障碍有关。
目的探索內皮素-1（ET-1）在肝髒缺血再灌註損傷中的作用。方法選用雄性Wistar大鼠80隻，分為正常對照組、缺血再灌註組、生理鹽水組和ET-1抗體組，觀察肝髒缺血60min再灌註3h後血漿ET-1、丙氨痠轉氨酶（ALT）、透明質痠（HA）、以及肝組織中ET-1和丙二醛（MDA）含量的變化，併觀察肝組織病理學變化，同時，在缺血再灌註組選擇第1、3、6、12和24h時相點觀察ET-1的變化規律。結果肝髒缺血再灌註後，血漿和肝組織中ET-1，血漿HA、ALT和肝組織中MDA顯著升高，而ET-1抗體組血漿ET-1、HA、ALT與缺血再灌註組相比顯著降低（P＜0.01,P＜0.05），同時，肝組織的瘀血程度和損傷程度顯著改善。結論 ET-1參與瞭肝髒缺血再灌註損傷，這種損傷與肝髒微循環障礙有關。
목적탐색내피소-1（ET-1）재간장결혈재관주손상중적작용。방법선용웅성Wistar대서80지，분위정상대조조、결혈재관주조、생리염수조화ET-1항체조，관찰간장결혈60min재관주3h후혈장ET-1、병안산전안매（ALT）、투명질산（HA）、이급간조직중ET-1화병이철（MDA）함량적변화，병관찰간조직병이학변화，동시，재결혈재관주조선택제1、3、6、12화24h시상점관찰ET-1적변화규률。결과간장결혈재관주후，혈장화간조직중ET-1，혈장HA、ALT화간조직중MDA현저승고，이ET-1항체조혈장ET-1、HA、ALT여결혈재관주조상비현저강저（P＜0.01,P＜0.05），동시，간조직적어혈정도화손상정도현저개선。결론 ET-1삼여료간장결혈재관주손상，저충손상여간장미순배장애유관。
Objective To investigate the effect of endothelin-1 (ET-1) on hepatic ischemia-reperfusion injury in rats.Methods Eighty male Wistar rats were randomly divided into the normal control, ischemia-reperfusion, physiologic saline and endothelin-1 antibody groups and killed at 3 rd h after ischemia induced for 60?min and reperfused for 3?h. plasma and(or) liver (ET-1), glutamic-pyruvic transaminase enzyme (ALT), hyaluronic acid (HA), malondialdehyde (MDA) were measured and the histopathologically hepatic alterations were observed. Meanwhile, the changes of plasma ET-1 were also observed at the time points of 1, 3, 6, 12 and 24?h in the ischemia-reperfusion group. Results Plasma and liver ET-1, Plasma HA, plasma ALT and liver MDA concentrations were markedly increased after hepatic ischemia-reperfusion, whereas the plasma ET-1, HA, ALT levels in ET-1 antibody group were significantly decreased compared with the ischemia-reperfusion group（P＜0.01, P＜0.05）. At the same time, the hepatic congestion and damages were markedly improved.Conclusion ET-1 might be participated in injury of the hepatic ischemia-reperfusion, which was correlated with the hepatic microcirculatory disturbances.
