CAJ | 학술논문

目的分离位于染色体3p24-26区域中鼻咽癌新的抑瘤基因。方法在染色体3p24-26鼻咽癌杂合性丢失(1oss of heterogosity，LOH)高频区，用RT-PCR及Northern杂交检测了20个表达序列标记(expressed sequence tag，EST)在鼻咽癌细胞株HNE-1和原代培养的正常鼻咽上皮细胞中的表达水平，并对其中一个在鼻咽癌细胞株HNE-1中表达下调的EST在19例鼻咽癌活检组织中的表达进行了检测。用cDNA文库筛选方法获得其全长cDNA序列，对该序列进行了初步分析。结果获得一个位于染色体3p25.3的新基因，命名为NAG-7基因。该基因在26.3％(5/19)的鼻咽癌活检组织中表达下调。它全长1667bp，其开放阅读框(open reading frame，ORF)编码一个含94个氨基酸的碱性蛋白质，分子量为11023.87道尔顿，预测为一跨膜蛋白质。它含有一蛋白激酶C磷酸化位点和肉豆蔻基位点。迄今为止，NAG-7基因序列在基因库中未见任何同源性基因。结论 NAG-7基因是一个在鼻咽癌中表达下调的新基因，它的改变可能参与了鼻咽癌的发生发展。
목적분리위우염색체3p24-26구역중비인암신적억류기인。방법재염색체3p24-26비인암잡합성주실(1oss of heterogosity，LOH)고빈구，용RT-PCR급Northern잡교검측료20개표체서렬표기(expressed sequence tag，EST)재비인암세포주HNE-1화원대배양적정상비인상피세포중적표체수평，병대기중일개재비인암세포주HNE-1중표체하조적EST재19례비인암활검조직중적표체진행료검측。용cDNA문고사선방법획득기전장cDNA서렬，대해서렬진행료초보분석。결과획득일개위우염색체3p25.3적신기인，명명위NAG-7기인。해기인재26.3％(5/19)적비인암활검조직중표체하조。타전장1667bp，기개방열독광(open reading frame，ORF)편마일개함94개안기산적감성단백질，분자량위11023.87도이돈，예측위일과막단백질。타함유일단백격매C린산화위점화육두구기위점。흘금위지，NAG-7기인서렬재기인고중미견임하동원성기인。결론 NAG-7기인시일개재비인암중표체하조적신기인，타적개변가능삼여료비인암적발생발전。
Objective To identify novel tumor suppressor genes at chromosome 3p24-26 in human nasopharyngeal carcinoma (NPC).Methods Twenty epithelial-derived expressed sequence tags (EST) were selected from chromosome 3p24-26. RT-PCR and Northern blot were used to detect the expression of the ESTs in NPC cell line, HNE-1, and primary cultures of normal nasopharyngeal epithelial cells. One EST, which was substantially downregulated in the HNE-1 cell line, was detected in 19 NPC biopsy samples, cDNA library screening was used to get its full sequence and the sequence of this novel gene was analyzed.Results A novel gene located at chromosome 3p25.3 was obtained and named NAG-7. It was downregulated in 26.3％ (5/19) of NPC biopsy samples. Its 1677 bp full length cDNA had a potential open reading frame predicting a 94 amino acid protein with a molecular weight of 11023.87 Dalton. Analysis of the NAG-7 gene showed that it was a transmembrane protein containing a protein kinase C phosphorylation site and a myristyl site. It has no significant homology to any reported genes in the database of GenBank. Conclusion NAG-7 is a novel gene downregulated in NPC, suggesting that it may be involved in the development of NPC.