CAJ | 학술논문

为充分利用雪龙兼松屠宰场废弃的优秀母牛卵巢，加速雪龙黑牛培育进程，满足高档牛肉市场需求，本文对胚胎生产体外受精3个关键技术环节进行了试验研究，共做了15批次试验，包括卵母细胞体外成熟培养的试验研究，卵母细胞体外受精试验研究以及受精卵体外培养试验研究。结果显示，卵母细胞的成熟率达98．6％，卵裂率为86．5％，囊胚发育率为36．8％。初步建立了一整套雪龙黑牛试管胚胎产业化生产培养方法和技术。
위충분이용설룡겸송도재장폐기적우수모우란소，가속설룡흑우배육진정，만족고당우육시장수구，본문대배태생산체외수정3개관건기술배절진행료시험연구，공주료15비차시험，포괄란모세포체외성숙배양적시험연구，란모세포체외수정시험연구이급수정란체외배양시험연구。결과현시，란모세포적성숙솔체98．6％，란렬솔위86．5％，낭배발육솔위36．8％。초보건립료일정투설룡흑우시관배태산업화생산배양방법화기술。
Three experiments were made to study oocyte of in vitro developed blastula for superiority beef cattle in world. The first was oocytes maturation. The second was fertilization in vitro. The third was developed blastula.The result indicated that the ratio ooeyte maturation was 98.6%, the cleavage ratio was 86.5%, the ratio blastula was 36.8%. A method to produce embryo of Xuelong Black Cattle in vitro was established.