CAJ | 학술논문

根据核rRNA基因ITS序列、叶绿体trnL-trnF和psbA-trnH序列对湘西宏成制药公司不同基地泡桐进行分子鉴定.用PCR从泡桐基因组DNA中分别扩增出大小约为500bp的ITS序列、540bp的psbA-trnH序列和900bp的trnL-trnF序列.相似性分析结果表明,毛泡桐ITS序列与1号和2号基地泡桐的相似性高于95%,与3号和4号基地和吉首大学校园泡桐的相似性低于79%;毛泡桐trnL-trnF序列与1号和2号基地泡桐的相似性均为99.5%,与3号和4号基地和吉首大学校园泡桐的相似性都低于70%;宏成制药4个基地和吉首大学校园泡桐psbA-trnH序列的相似性都高于99%.说明1号和2号基地泡桐属于毛泡桐,3号和4号基地和吉首大学校园泡桐属于白花泡桐.
근거핵rRNA기인ITS서렬、협록체trnL-trnF화psbA-trnH서렬대상서굉성제약공사불동기지포동진행분자감정.용PCR종포동기인조DNA중분별확증출대소약위500bp적ITS서렬、540bp적psbA-trnH서렬화900bp적trnL-trnF서렬.상사성분석결과표명,모포동ITS서렬여1호화2호기지포동적상사성고우95%,여3호화4호기지화길수대학교완포동적상사성저우79%;모포동trnL-trnF서렬여1호화2호기지포동적상사성균위99.5%,여3호화4호기지화길수대학교완포동적상사성도저우70%;굉성제약4개기지화길수대학교완포동psbA-trnH서렬적상사성도고우99%.설명1호화2호기지포동속우모포동,3호화4호기지화길수대학교완포동속우백화포동.
The genomic DNA was isolated from leaves of Paulownia plants through the CTAB method without liquid nitrogen.The internal transcribed spacer（ITS） of rRNA and two intergenic spacers of psbA-trnH and trnF-trnF were amplified from the genomic DNA by PCR and then sequenced.Sequence analysis of the three DNA markers were conducted with software Clustal X7.0,and their phylogenetic trees were constructed with software MEGA3.1.The results showed three fragments of ITS,psbA-trnH and trnL-trnF was 600 bp,540 bp and 900 bp in length,respectively.Similarity of ITS sequence between the previously reported Paulownia tomentosa and Paulownia No.1 or No.2 was higher than 95%,but that between the P.tomentosa and No.3,No.4 or No.5 of Paulownia was less than 79%.Similarity of the trnL-trnF between the P.tomentosa and No.1 or No.2 of Paulownia both was 99.5%,and that between the P.tomentosa and No.3,No.4 or No.5 of Paulownia was less than 70%.The results indicated that sequence of the psbA-trnH of Paulownia species in different cultivation places was very conservative and it could not provide enough information for the relationship of the species among Paulownia.Molecular identification demonstrated that Paulownia No.1 and No.2 represented P.tomentosa,and Paulownia No.3,No.4 and No.5 represented P.fortunei.