CAJ | 학술논문

以鲤鱼鱼皮为研究对象,通过碱溶、酸溶、NaCl盐析等方法从鱼皮中分离纯化得到了胶原蛋白.SDS-PAGE分析显示,鲤鱼胶原蛋白由分子质量约为120 ku和115 ku的2条α链（α1与α2）、分子质量约为250 ku的β链以及大分子质量的γ链组成.分析比较鲤鱼胶原蛋白与鲢鱼小清蛋白的基本性质,结果显示：2种蛋白质的pH值稳定性相似;胶原蛋白的热稳定性不如小清蛋白,但比小清蛋白更耐胃蛋白酶消化;在高温高压处理下,胶原蛋白明显降解,小清蛋白则出现了降解与聚合2种情况.应用8份鱼类过敏患者血清的酶联免疫吸附测定实验结果显示,小清蛋白对6份血清有特异性IgE反应,胶原蛋白对3份血清有特异性IgE反应.Dot-blot实验结果显示,胶原蛋白与鱼类过敏患者血清出现了特异性的杂交显色斑点.与小清蛋白相似,鲤鱼胶原蛋白具有特异的IgE结合活性,提示其是一种潜在的过敏原.
이리어어피위연구대상,통과감용、산용、NaCl염석등방법종어피중분리순화득도료효원단백.SDS-PAGE분석현시,리어효원단백유분자질량약위120 ku화115 ku적2조α련（α1여α2）、분자질량약위250 ku적β련이급대분자질량적γ련조성.분석비교리어효원단백여련어소청단백적기본성질,결과현시：2충단백질적pH치은정성상사;효원단백적열은정성불여소청단백,단비소청단백경내위단백매소화;재고온고압처리하,효원단백명현강해,소청단백칙출현료강해여취합2충정황.응용8빈어류과민환자혈청적매련면역흡부측정실험결과현시,소청단백대6빈혈청유특이성IgE반응,효원단백대3빈혈청유특이성IgE반응.Dot-blot실험결과현시,효원단백여어류과민환자혈청출현료특이성적잡교현색반점.여소청단백상사,리어효원단백구유특이적IgE결합활성,제시기시일충잠재적과민원.
Collagen of carp skin was isolated by alkali extraction,acid extraction and NaCl precipitation.Carp collagen consisted of α1 chain（120 ku）,α2 chain（115ku）,β chain（250 ku）,and small amount of γ chain in SDS-PAGE.Analysis of the basic property of collagen and parvalbumin（the major allergen of fish）showed the pH stability of two proteins did not present distinct difference;collagen was less stable to heating treatment,but more resistive to SGF digestion;treated with high temperature and high pressure,collagen was decomposed into fragments,parvalbumin presented decomposition and polymerization simultaneously.ELISA analysis of eight sera from fish-allergic patients showed parvalbumin had the specific IgE reactivity to six sera,while collagen had the specific IgE reactivity to three sera.Dot-blot analysis of collagen also presented the specific IgE reactivity to the sera from fish-allergic patients.The results indicated that carp collagen might be a potential allergen.