CAJ | 학술논문

根据GenBank中公布的鸭GH基因序列,设计4对引物,以黑番鸭血液基因组DNA为模板,采用PCR方法,扩增出GH基因4650bp的DNA序列,该序列包含了完整的内含子和外显子序列,由5个外显子和4个内含子组成。进一步将克隆的黑番鸭GH基因编码的氨基酸序列与GenBank中其他物种的该基因氨基酸序列进行同源性分析和聚类分析,结果表明：黑番鸭与其他禽类的同源性为91.2%-98.5%。同属雁形目鸭科的黑番鸭、鹅、和鸵鸟亲缘关系较近,聚为一大类;同属鸡形目雉科的家鸡、鹌鹑和火鸡等则聚为一大类;另外,小鼠和猪聚为一类。
근거GenBank중공포적압GH기인서렬,설계4대인물,이흑번압혈액기인조DNA위모판,채용PCR방법,확증출GH기인4650bp적DNA서렬,해서렬포함료완정적내함자화외현자서렬,유5개외현자화4개내함자조성。진일보장극륭적흑번압GH기인편마적안기산서렬여GenBank중기타물충적해기인안기산서렬진행동원성분석화취류분석,결과표명：흑번압여기타금류적동원성위91.2%-98.5%。동속안형목압과적흑번압、아、화타조친연관계교근,취위일대류;동속계형목치과적가계、암순화화계등칙취위일대류;령외,소서화저취위일류。
According to the GenBank sequences of the duck GH gene,four pairs of specific primers were designed.The length of 4 650 bp DNA sequence was amplified from the genomic DNA abstracted from black muscovy duck blood by PCR technique.The sequences included 4 introns and 5 exons.The result of homology analysis and clustering analysis indicated that the homology had 91.2%～98.5% similarity between black muscovy duck and other birds.The result of clustering analysis showed black muscovy duck,goose and ostich clustered a class,chick,quail and turkey clustered a class,the mouse and pig clustered a class.